Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5212194..5212770 Replicon chromosome
Accession NZ_CP099306
Organism Klebsiella michiganensis strain RHB20-SO-C01

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag NFK05_RS24545 Protein ID WP_064375702.1
Coordinates 5212483..5212770 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag NFK05_RS24540 Protein ID WP_014227757.1
Coordinates 5212194..5212496 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK05_RS24525 (NFK05_24500) 5209035..5209370 + 336 WP_064375703.1 endoribonuclease SymE -
NFK05_RS24530 (NFK05_24505) 5209822..5210733 + 912 WP_181251267.1 acetamidase/formamidase family protein -
NFK05_RS24535 (NFK05_24510) 5210730..5212073 + 1344 WP_049101852.1 APC family permease -
NFK05_RS24540 (NFK05_24515) 5212194..5212496 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
NFK05_RS24545 (NFK05_24520) 5212483..5212770 - 288 WP_064375702.1 BrnT family toxin Toxin
NFK05_RS24550 (NFK05_24525) 5213018..5213461 - 444 WP_064375701.1 FosA family fosfomycin resistance glutathione transferase -
NFK05_RS24555 (NFK05_24530) 5213455..5214363 - 909 WP_064375700.1 LysR family transcriptional regulator -
NFK05_RS24560 (NFK05_24535) 5214451..5215233 + 783 WP_181251268.1 NAD(P)H-dependent oxidoreductase -
NFK05_RS24565 (NFK05_24540) 5215381..5215965 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
NFK05_RS24570 (NFK05_24545) 5216111..5216911 + 801 WP_064375698.1 helix-turn-helix domain-containing protein -
NFK05_RS24575 (NFK05_24550) 5216908..5217426 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11210.69 Da        Isoelectric Point: 7.4687

>T248367 WP_064375702.1 NZ_CP099306:c5212770-5212483 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT248367 WP_014227757.1 NZ_CP099306:c5212496-5212194 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References