Detailed information of TA system TA024836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2645788..2646455
• Replicon: chromosome
• Accession: NC_014315
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D8K9B3
• Locus tag: NWAT_RS11970
• Protein ID: WP_013221326.1
• Coordinates: 2645788..2646207 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D8K9B4
• Locus tag: NWAT_RS11975
• Protein ID: WP_013221327.1
• Coordinates: 2646204..2646455 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS11950	2641208..2641581	+	374	Protein_2369	hypothetical protein	-
NWAT_RS11955	2641591..2643141	+	1551	WP_013221322.1	DUF262 domain-containing protein	-
NWAT_RS11960	2643102..2645129	+	2028	WP_083781452.1	DUF262 domain-containing HNH endonuclease family protein	-
NWAT_RS16745	2645162..2645314	-	153	WP_157679906.1	hypothetical protein	-
NWAT_RS16750	2645620..2645772	+	153	WP_013221325.1	resolvase domain-containing protein	-
NWAT_RS11970	2645788..2646207	-	420	WP_013221326.1	type II toxin-antitoxin system VapC family toxin	Toxin
NWAT_RS11975	2646204..2646455	-	252	WP_013221327.1	Arc family DNA-binding protein	Antitoxin
NWAT_RS11980	2646722..2647273	+	552	WP_013221328.1	recombinase family protein	-
NWAT_RS16240	2647415..2647649	+	235	Protein_2377	helix-turn-helix domain-containing protein	-
NWAT_RS11985	2647686..2648039	+	354	WP_013221329.1	IS6 family transposase	-
NWAT_RS11990	2648335..2649795	+	1461	WP_041350725.1	DUF262 domain-containing protein	-
NWAT_RS11995	2649847..2650551	+	705	WP_013221331.1	hypothetical protein	-
NWAT_RS16520	2650798..2651066	+	269	Protein_2381	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2639403..2648039	8636
	-	flank	IS/Tn	-	-	2646722..2647273	551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14984.32 Da
• Isoelectric Point: 8.4725

>T24836 WP_013221326.1 NC_014315:c2646207-2645788 [Nitrosococcus watsonii C-113]
VILVDTNVISEPLRKAPEARVIAWMDAQPLETLYLSAITIAELRFGVASLPAGKRRDRLYESLERQVLPLFTNRVLSFDL
AASRTYAALMAKARAAGLAIGVSDGYIAATATAHGMRVATRDTAPFEAAGVSIINPWEA

Nucleotide

• Length: 420 bp

>T24836 NC_014315:c2646207-2645788 [Nitrosococcus watsonii C-113]
GTGATTCTGGTCGACACGAATGTGATTTCGGAGCCACTGCGCAAGGCGCCCGAGGCCCGCGTCATCGCGTGGATGGACGC
CCAGCCGCTCGAAACGCTATACCTATCGGCCATTACCATTGCCGAGCTGCGTTTCGGTGTGGCGTCGCTACCGGCTGGCA
AGCGGCGGGATAGACTGTATGAGAGCCTGGAGCGGCAAGTGTTGCCGCTGTTCACCAACCGCGTACTGTCGTTCGACCTA
GCGGCCTCCCGGACCTATGCCGCGCTGATGGCGAAGGCACGCGCGGCCGGGTTGGCGATTGGGGTGAGCGATGGATATAT
CGCAGCAACGGCGACCGCCCATGGGATGAGGGTGGCCACCAGGGACACAGCACCGTTTGAGGCCGCGGGCGTGTCGATCA
TCAATCCGTGGGAGGCGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9279.66 Da
• Isoelectric Point: 9.9880

>AT24836 WP_013221327.1 NC_014315:c2646455-2646204 [Nitrosococcus watsonii C-113]
MAMLTVRNLPEEVHRALRVRAATHGRSTEAEVRAILEEAVKPEGRVKLGSLLAGIGRRAGLKDEEYAVFEQRDKTPAQPV
RFE

Nucleotide

• Length: 252 bp

>AT24836 NC_014315:c2646455-2646204 [Nitrosococcus watsonii C-113]
ATGGCCATGCTGACAGTTCGCAACCTACCCGAAGAAGTGCACCGCGCCCTACGCGTGCGAGCCGCCACGCACGGGCGCAG
CACCGAAGCTGAGGTGCGTGCCATCCTGGAAGAGGCGGTAAAGCCGGAAGGGCGGGTTAAGCTCGGTTCCCTGCTGGCCG
GCATTGGTCGGCGTGCTGGGCTCAAGGACGAGGAGTATGCGGTCTTTGAGCAGCGCGACAAGACCCCCGCCCAGCCGGTC
CGCTTTGAGTGA

Structures

Toxin

• AlphaFold DB: D8K9B3

Antitoxin

• AlphaFold DB: D8K9B4