Detailed information of TA system TA024835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2636087..2636582
• Replicon: chromosome
• Accession: NC_014315
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: -
• Uniprot ID: D8K9A3
• Locus tag: NWAT_RS11900
• Protein ID: WP_013221316.1
• Coordinates: 2636087..2636350 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D8K9A4
• Locus tag: NWAT_RS11905
• Protein ID: WP_013221317.1
• Coordinates: 2636340..2636582 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS11875	2632352..2632591	+	240	WP_041350714.1	hypothetical protein	-
NWAT_RS11880	2632869..2635031	+	2163	WP_157679900.1	chromosome segregation protein SMC	-
NWAT_RS11885	2635039..2635458	+	420	WP_013221313.1	hypothetical protein	-
NWAT_RS11890	2635485..2635700	+	216	WP_157679902.1	hypothetical protein	-
NWAT_RS11895	2635704..2635967	-	264	WP_198342213.1	WGR domain-containing protein	-
NWAT_RS11900	2636087..2636350	+	264	WP_013221316.1	BrnT family toxin	Toxin
NWAT_RS11905	2636340..2636582	+	243	WP_013221317.1	hypothetical protein	Antitoxin
NWAT_RS15710	2636736..2637197	+	462	WP_013221318.1	hypothetical protein	-
NWAT_RS11920	2637362..2639227	+	1866	WP_013221319.1	hypothetical protein	-
NWAT_RS11925	2639403..2639954	-	552	WP_013221320.1	recombinase family protein	-
NWAT_RS11930	2640044..2640307	+	264	WP_157679904.1	DUF4158 domain-containing protein	-
NWAT_RS17095	2640304..2640684	+	381	WP_041350719.1	hypothetical protein	-
NWAT_RS16235	2640725..2640994	-	270	Protein_2368	endonuclease	-
NWAT_RS11950	2641208..2641581	+	374	Protein_2369	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10084.54 Da
• Isoelectric Point: 8.5234

>T24835 WP_013221316.1 NC_014315:2636087-2636350 [Nitrosococcus watsonii C-113]
MHVFTYDEAKSQANLKKHGIDFLAAQALWADPDALEVRAKSDDEPRFIVIGLIDGRHWSAVVTYREDKIRLISVRRSRKK
EVLLYES

Nucleotide

• Length: 264 bp

>T24835 NC_014315:2636087-2636350 [Nitrosococcus watsonii C-113]
ATGCACGTGTTCACGTATGACGAAGCGAAAAGCCAAGCCAACCTGAAGAAACACGGTATCGATTTTCTTGCGGCACAGGC
GTTATGGGCCGATCCTGACGCCCTGGAAGTGCGGGCAAAATCAGATGACGAGCCCCGATTCATTGTGATTGGCCTTATAG
ACGGGCGGCATTGGTCTGCTGTTGTCACGTATCGCGAAGATAAAATTCGCCTGATTTCCGTCAGGCGTTCACGTAAAAAA
GAGGTGCTGCTCTATGAAAGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9333.62 Da
• Isoelectric Point: 9.9441

>AT24835 WP_013221317.1 NC_014315:2636340-2636582 [Nitrosococcus watsonii C-113]
MKAKDFDKKFDRGKEDIVDELDLSTMRRPNQEQKRINVDFPSWVVDSLDREAARIGVTRQSIIKVWLVERLRSEAANKSG

Nucleotide

• Length: 243 bp

>AT24835 NC_014315:2636340-2636582 [Nitrosococcus watsonii C-113]
ATGAAAGCTAAAGACTTCGACAAAAAGTTTGACCGGGGTAAAGAGGATATAGTTGATGAACTTGATTTGTCGACTATGCG
TCGTCCTAATCAGGAGCAAAAGCGAATTAACGTTGATTTCCCCTCTTGGGTTGTTGATTCGCTTGATCGCGAAGCGGCAC
GCATAGGCGTTACTCGCCAGTCCATTATTAAAGTCTGGTTGGTCGAGCGCTTGCGCTCTGAAGCGGCTAACAAGTCTGGT
TAG

Structures

Toxin

• AlphaFold DB: D8K9A3

Antitoxin

• AlphaFold DB: D8K9A4