Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2080607..2081197 Replicon chromosome
Accession NZ_CP099304
Organism Klebsiella michiganensis strain RHB20-SO-C02

Toxin (Protein)


Gene name pemK Uniprot ID A0A7H5AAK0
Locus tag NFK06_RS09725 Protein ID WP_004852304.1
Coordinates 2080865..2081197 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag NFK06_RS09720 Protein ID WP_004852307.1
Coordinates 2080607..2080864 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK06_RS09710 (NFK06_09710) 2079915..2080224 + 310 Protein_1904 hypothetical protein -
NFK06_RS09715 (NFK06_09715) 2080227..2080346 + 120 Protein_1905 helix-turn-helix domain-containing protein -
NFK06_RS09720 (NFK06_09720) 2080607..2080864 + 258 WP_004852307.1 antitoxin Antitoxin
NFK06_RS09725 (NFK06_09725) 2080865..2081197 + 333 WP_004852304.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NFK06_RS09735 (NFK06_09735) 2081520..2082977 + 1458 WP_049079573.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
NFK06_RS09745 (NFK06_09745) 2083941..2085395 - 1455 WP_134897790.1 AMP nucleosidase -
NFK06_RS09750 (NFK06_09750) 2085528..2085785 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11863.81 Da        Isoelectric Point: 10.5834

>T248349 WP_004852304.1 NZ_CP099304:2080865-2081197 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTLGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT248349 WP_004852307.1 NZ_CP099304:2080607-2080864 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5AAK0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References