Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4622809..4623425 Replicon chromosome
Accession NZ_CP099303
Organism Citrobacter freundii strain RHB02-E1-C01

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag NFJ16_RS21980 Protein ID WP_003028682.1
Coordinates 4622809..4623183 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag NFJ16_RS21985 Protein ID WP_043018956.1
Coordinates 4623183..4623425 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ16_RS21965 (4620312) 4620312..4621214 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
NFJ16_RS21970 (4621211) 4621211..4621846 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFJ16_RS21975 (4621843) 4621843..4622772 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFJ16_RS21980 (4622809) 4622809..4623183 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
NFJ16_RS21985 (4623183) 4623183..4623425 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
NFJ16_RS21990 (4623631) 4623631..4624539 + 909 WP_003847901.1 alpha/beta hydrolase -
NFJ16_RS21995 (4624690) 4624690..4625631 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NFJ16_RS22000 (4625676) 4625676..4626113 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
NFJ16_RS22005 (4626110) 4626110..4626982 - 873 WP_003028669.1 virulence factor BrkB family protein -
NFJ16_RS22010 (4626976) 4626976..4627575 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T248346 WP_003028682.1 NZ_CP099303:c4623183-4622809 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT248346 WP_043018956.1 NZ_CP099303:c4623425-4623183 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References