Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3520299..3520919 Replicon chromosome
Accession NZ_CP099303
Organism Citrobacter freundii strain RHB02-E1-C01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag NFJ16_RS16885 Protein ID WP_002892050.1
Coordinates 3520701..3520919 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag NFJ16_RS16880 Protein ID WP_003021733.1
Coordinates 3520299..3520673 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ16_RS16870 (3515445) 3515445..3516638 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
NFJ16_RS16875 (3516661) 3516661..3519810 + 3150 WP_003021736.1 efflux RND transporter permease AcrB -
NFJ16_RS16880 (3520299) 3520299..3520673 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
NFJ16_RS16885 (3520701) 3520701..3520919 + 219 WP_002892050.1 HHA domain-containing protein Toxin
NFJ16_RS16890 (3521226) 3521226..3521777 + 552 WP_032936967.1 maltose O-acetyltransferase -
NFJ16_RS16895 (3521894) 3521894..3522364 + 471 WP_003021724.1 YlaC family protein -
NFJ16_RS16900 (3522443) 3522443..3522583 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
NFJ16_RS16905 (3522585) 3522585..3522845 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
NFJ16_RS16910 (3523034) 3523034..3524587 + 1554 WP_048241431.1 EAL domain-containing protein -
NFJ16_RS16915 (3524639) 3524639..3524992 - 354 WP_003831033.1 DUF1428 family protein -
NFJ16_RS16920 (3525057) 3525057..3525686 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T248341 WP_002892050.1 NZ_CP099303:3520701-3520919 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT248341 WP_003021733.1 NZ_CP099303:3520299-3520673 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References