Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4921175..4921791 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag NFJ20_RS23675 Protein ID WP_003028682.1
Coordinates 4921175..4921549 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag NFJ20_RS23680 Protein ID WP_043018956.1
Coordinates 4921549..4921791 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS23660 (4918678) 4918678..4919580 + 903 WP_016155183.1 formate dehydrogenase O subunit beta -
NFJ20_RS23665 (4919577) 4919577..4920212 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFJ20_RS23670 (4920209) 4920209..4921138 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFJ20_RS23675 (4921175) 4921175..4921549 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
NFJ20_RS23680 (4921549) 4921549..4921791 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
NFJ20_RS23685 (4921997) 4921997..4922905 + 909 WP_046671350.1 alpha/beta hydrolase -
NFJ20_RS23690 (4923056) 4923056..4923997 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NFJ20_RS23695 (4924042) 4924042..4924479 - 438 WP_003840439.1 D-aminoacyl-tRNA deacylase -
NFJ20_RS23700 (4924476) 4924476..4925345 - 870 WP_136397728.1 virulence factor BrkB family protein -
NFJ20_RS23705 (4925339) 4925339..4925938 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T248332 WP_003028682.1 NZ_CP099298:c4921549-4921175 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT248332 WP_043018956.1 NZ_CP099298:c4921791-4921549 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References