Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4520016..4520636 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag NFJ20_RS21715 Protein ID WP_002892050.1
Coordinates 4520016..4520234 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag NFJ20_RS21720 Protein ID WP_003021733.1
Coordinates 4520262..4520636 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS21680 (4515249) 4515249..4515878 + 630 WP_279274121.1 hypothetical protein -
NFJ20_RS21685 (4515943) 4515943..4516296 + 354 WP_003831033.1 DUF1428 family protein -
NFJ20_RS21690 (4516348) 4516348..4517901 - 1554 WP_003835927.1 EAL domain-containing protein -
NFJ20_RS21695 (4518090) 4518090..4518350 + 261 WP_003835926.1 type B 50S ribosomal protein L31 -
NFJ20_RS21700 (4518352) 4518352..4518492 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
NFJ20_RS21705 (4518571) 4518571..4519041 - 471 WP_003021724.1 YlaC family protein -
NFJ20_RS21710 (4519158) 4519158..4519709 - 552 WP_203398439.1 maltose O-acetyltransferase -
NFJ20_RS21715 (4520016) 4520016..4520234 - 219 WP_002892050.1 HHA domain-containing protein Toxin
NFJ20_RS21720 (4520262) 4520262..4520636 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
NFJ20_RS21725 (4521125) 4521125..4524274 - 3150 WP_003021736.1 efflux RND transporter permease AcrB -
NFJ20_RS21730 (4524297) 4524297..4525490 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T248330 WP_002892050.1 NZ_CP099298:c4520234-4520016 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT248330 WP_003021733.1 NZ_CP099298:c4520636-4520262 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References