Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3065425..3066015 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag NFJ20_RS14830 Protein ID WP_181825620.1
Coordinates 3065425..3065757 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag NFJ20_RS14835 Protein ID WP_003836694.1
Coordinates 3065758..3066015 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS14795 (3060711) 3060711..3061376 + 666 WP_279273855.1 hypothetical protein -
NFJ20_RS14800 (3061334) 3061334..3061735 + 402 WP_279273856.1 SPASM domain-containing protein -
NFJ20_RS14805 (3062154) 3062154..3062510 + 357 WP_279273857.1 hypothetical protein -
NFJ20_RS14810 (3062648) 3062648..3062836 + 189 WP_231501072.1 helix-turn-helix domain-containing protein -
NFJ20_RS14815 (3062981) 3062981..3063931 - 951 WP_003836688.1 HTH-type transcriptional regulator Cbl -
NFJ20_RS14820 (3064033) 3064033..3064950 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
NFJ20_RS14830 (3065425) 3065425..3065757 - 333 WP_181825620.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NFJ20_RS14835 (3065758) 3065758..3066015 - 258 WP_003836694.1 hypothetical protein Antitoxin
NFJ20_RS14840 (3066630) 3066630..3070529 + 3900 WP_279273858.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3053361..3076398 23037


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11791.54 Da        Isoelectric Point: 7.8365

>T248328 WP_181825620.1 NZ_CP099298:c3065757-3065425 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARTAGFTVSLDDAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT248328 WP_003836694.1 NZ_CP099298:c3066015-3065758 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References