Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1626918..1628541 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name hipA Uniprot ID A0A7D6U035
Locus tag NFJ20_RS07750 Protein ID WP_020833804.1
Coordinates 1627216..1628541 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5V9NJV0
Locus tag NFJ20_RS07745 Protein ID WP_024143021.1
Coordinates 1626918..1627211 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS07730 (1622260) 1622260..1623852 + 1593 WP_039264109.1 IS66 family transposase -
NFJ20_RS07740 (1625249) 1625249..1626517 + 1269 WP_020833806.1 hypothetical protein -
NFJ20_RS07745 (1626918) 1626918..1627211 + 294 WP_024143021.1 helix-turn-helix transcriptional regulator Antitoxin
NFJ20_RS07750 (1627216) 1627216..1628541 + 1326 WP_020833804.1 type II toxin-antitoxin system HipA family toxin Toxin
NFJ20_RS07755 (1628602) 1628602..1628808 + 207 WP_000134171.1 hypothetical protein -
NFJ20_RS07760 (1628996) 1628996..1629472 + 477 WP_230204012.1 TnsD family Tn7-like transposition protein -
NFJ20_RS07765 (1629472) 1629472..1631070 + 1599 WP_020833803.1 Tn7-like element transposition protein TnsE -
NFJ20_RS07770 (1631234) 1631234..1632136 + 903 WP_020833802.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50307.23 Da        Isoelectric Point: 6.0252

>T248322 WP_020833804.1 NZ_CP099298:1627216-1628541 [Citrobacter freundii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10990.39 Da        Isoelectric Point: 9.9492

>AT248322 WP_024143021.1 NZ_CP099298:1626918-1627211 [Citrobacter freundii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKLNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6U035

Antitoxin

Source ID Structure
AlphaFold DB A0A5V9NJV0

References