Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1620695..1621221 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NFJ20_RS07715 Protein ID WP_000323025.1
Coordinates 1620934..1621221 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NFJ20_RS07710 Protein ID WP_000534858.1
Coordinates 1620695..1620934 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS07690 (1617240) 1617240..1617812 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
NFJ20_RS07695 (1618012) 1618012..1618935 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
NFJ20_RS07700 (1619101) 1619101..1620339 - 1239 WP_279274374.1 IS110 family transposase -
NFJ20_RS07705 (1620572) 1620572..1620670 - 99 Protein_1502 protein YdfV -
NFJ20_RS07710 (1620695) 1620695..1620934 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NFJ20_RS07715 (1620934) 1620934..1621221 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NFJ20_RS07720 (1621475) 1621475..1621882 + 408 WP_021567585.1 transposase -
NFJ20_RS07725 (1621879) 1621879..1622229 + 351 WP_021567584.1 IS66 family insertion sequence element accessory protein TnpB -
NFJ20_RS07730 (1622260) 1622260..1623852 + 1593 WP_039264109.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T248321 WP_000323025.1 NZ_CP099298:1620934-1621221 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT248321 WP_000534858.1 NZ_CP099298:1620695-1620934 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References