248314

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 4687551..4688217 Replicon chromosome
Accession NZ_CP099291
Organism Citrobacter freundii strain RHB03-E1-C03

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag NFJ31_RS22615 Protein ID WP_044702260.1
Coordinates 4687858..4688217 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag NFJ31_RS22610 Protein ID WP_003844682.1
Coordinates 4687551..4687868 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ31_RS22580 (4683218) 4683218..4684042 + 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
NFJ31_RS22585 (4684111) 4684111..4685334 + 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
NFJ31_RS22590 (4685336) 4685336..4686148 + 813 WP_044702264.1 shikimate 5-dehydrogenase -
NFJ31_RS22595 (4686200) 4686200..4686556 - 357 WP_032948294.1 hypothetical protein -
NFJ31_RS22600 (4686698) 4686698..4686859 + 162 WP_003841416.1 phage protein -
NFJ31_RS22605 (4687263) 4687263..4687541 + 279 WP_044702262.1 putative addiction module antidote protein -
NFJ31_RS22610 (4687551) 4687551..4687868 - 318 WP_003844682.1 helix-turn-helix transcriptional regulator Antitoxin
NFJ31_RS22615 (4687858) 4687858..4688217 - 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFJ31_RS22620 (4688431) 4688431..4689120 + 690 WP_003841421.1 dipeptidase PepE -
NFJ31_RS22625 (4689186) 4689186..4690817 - 1632 WP_032938230.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T248314 WP_044702260.1 NZ_CP099291:c4688217-4687858 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT248314 WP_003844682.1 NZ_CP099291:c4687868-4687551 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References