Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3981918..3982597 Replicon chromosome
Accession NZ_CP099291
Organism Citrobacter freundii strain RHB03-E1-C03

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag NFJ31_RS19350 Protein ID WP_003031349.1
Coordinates 3982256..3982597 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag NFJ31_RS19345 Protein ID WP_003031347.1
Coordinates 3981918..3982235 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ31_RS19320 (3978011) 3978011..3980008 - 1998 WP_016245729.1 choline BCCT transporter BetT -
NFJ31_RS19325 (3980556) 3980556..3980703 + 148 Protein_3789 hypothetical protein -
NFJ31_RS19330 (3980717) 3980717..3981178 + 462 WP_003031344.1 antirestriction protein -
NFJ31_RS19335 (3981194) 3981194..3981670 + 477 WP_003031345.1 RadC family protein -
NFJ31_RS19340 (3981679) 3981679..3981900 + 222 WP_003031346.1 DUF987 domain-containing protein -
NFJ31_RS19345 (3981918) 3981918..3982235 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
NFJ31_RS19350 (3982256) 3982256..3982597 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
NFJ31_RS19360 (3983227) 3983227..3983535 - 309 WP_230132591.1 isoprenylcysteine carboxylmethyltransferase family protein -
NFJ31_RS19365 (3983883) 3983883..3985331 - 1449 WP_044702495.1 EAL domain-containing protein -
NFJ31_RS19370 (3985673) 3985673..3986146 - 474 WP_044702484.1 hypothetical protein -
NFJ31_RS19375 (3986121) 3986121..3986852 - 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3967545..3994493 26948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T248311 WP_003031349.1 NZ_CP099291:3982256-3982597 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT248311 WP_003031347.1 NZ_CP099291:3981918-3982235 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References