Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 32839..33749 Replicon plasmid unnamed1
Accession NZ_CP099261
Organism Klebsiella aerogenes strain RHB08-SO-C04

Toxin (Protein)


Gene name - Uniprot ID -
Locus tag NFJ54_RS25910 Protein ID WP_112029140.1
Coordinates 33279..33749 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag NFJ54_RS25905 Protein ID WP_004026357.1
Coordinates 32839..33282 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ54_RS25875 (NFJ54_25840) 28334..28531 + 198 Protein_31 hypothetical protein -
NFJ54_RS25880 (NFJ54_25845) 28534..29079 - 546 WP_112029143.1 winged helix DNA-binding protein -
NFJ54_RS25885 (NFJ54_25850) 29278..30000 + 723 WP_086523242.1 DUF969 domain-containing protein -
NFJ54_RS25890 (NFJ54_25855) 29997..30983 + 987 WP_112029142.1 DUF979 domain-containing protein -
NFJ54_RS25895 (NFJ54_25860) 30993..31988 + 996 WP_112029141.1 DUF2891 domain-containing protein -
NFJ54_RS25900 (NFJ54_25865) 32149..32782 + 634 Protein_36 DUF4113 domain-containing protein -
NFJ54_RS25905 (NFJ54_25870) 32839..33282 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
NFJ54_RS25910 (NFJ54_25875) 33279..33749 + 471 WP_112029140.1 RES family NAD+ phosphorylase Toxin
NFJ54_RS25915 (NFJ54_25880) 33859..34119 - 261 WP_112029139.1 hypothetical protein -
NFJ54_RS25920 (NFJ54_25885) 34478..36223 - 1746 WP_112029138.1 biotin carboxylase N-terminal domain-containing protein -
NFJ54_RS25925 (NFJ54_25890) 36223..37806 - 1584 WP_112029137.1 urea amidolyase family protein -
NFJ54_RS25930 (NFJ54_25895) 37819..38625 - 807 WP_112029136.1 putative hydro-lyase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..183022 183022
- inside IScluster/Tn - - 21916..28276 6360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17591.95 Da        Isoelectric Point: 4.7424

>T248301 WP_112029140.1 NZ_CP099261:33279-33749 [Klebsiella aerogenes]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSINVPDELIQSANMD
ELPVNWADEDAPRELADYGDAWSFTRTSVALRVPSALSPVEFNYLLNPEHSEFYGIVQMAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT248301 WP_004026357.1 NZ_CP099261:32839-33282 [Klebsiella aerogenes]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References