Detailed information of TA system TA024828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 294503..295542
• Replicon: plasmid mpPSI07
• Accession: NC_014310
• Organism: Ralstonia solanacearum PSI07

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: RPSI07_RS01075
• Protein ID: WP_013209333.1
• Coordinates: 294967..295542 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: RPSI07_RS01070
• Protein ID: WP_013209332.1
• Coordinates: 294503..294970 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPSI07_RS01060	291007..292281	-	1275	WP_013209329.1	TIGR02678 family protein	-
RPSI07_RS01065	292278..293795	-	1518	WP_048816953.1	TIGR02677 family protein	-
RPSI07_RS01070	294503..294970	+	468	WP_013209332.1	helix-turn-helix domain-containing protein	Antitoxin
RPSI07_RS01075	294967..295542	+	576	WP_013209333.1	PIN domain-containing protein	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..2085000	2085000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21654.87 Da
• Isoelectric Point: 5.6294

>T24828 WP_013209333.1 NC_014310:294967-295542 [Ralstonia solanacearum PSI07]
MRHSPFTAIYDACVLYPAPLRDFLMWLGLSGRFRARWSQNIHEEWKRNLLLNRPDLSREQLDRTSAFMDRAIPDGLVEGY
EELIEGLVLPDPGDRHVLAAAIRCGASVIVTFNQKDFPEAALAPFGIEAQHPDEFVDNLFDLDAAAIVAAARRQRAHLVN
PPMETDRYLEMLLRQGLVQTAKALGTYRLIL

Nucleotide

• Length: 576 bp

>T24828 NC_014310:294967-295542 [Ralstonia solanacearum PSI07]
ATGAGGCATTCCCCCTTCACGGCAATCTACGACGCATGCGTGTTGTATCCCGCTCCGTTGCGAGACTTCCTGATGTGGCT
GGGTCTGTCCGGGAGGTTTCGCGCGCGATGGAGTCAGAACATCCATGAGGAATGGAAGCGCAATTTGCTCTTGAACCGGC
CGGATCTGAGTCGCGAGCAGTTGGATCGGACTTCGGCGTTCATGGATCGGGCCATTCCCGACGGGCTGGTTGAGGGATAT
GAGGAATTGATCGAGGGCTTGGTGTTGCCTGATCCGGGTGACCGGCATGTCCTGGCCGCGGCCATCCGCTGTGGCGCGAG
CGTCATCGTCACGTTCAATCAAAAAGATTTTCCGGAGGCTGCGCTAGCCCCCTTTGGAATTGAGGCGCAGCACCCCGACG
AGTTCGTGGACAACCTATTTGATCTGGATGCGGCAGCTATCGTCGCTGCCGCACGGCGGCAACGCGCTCACCTCGTCAAT
CCACCCATGGAGACGGACCGCTATCTGGAGATGCTACTCAGGCAAGGCTTGGTCCAGACAGCAAAGGCGTTGGGTACCTA
CCGGCTGATCCTCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17232.52 Da
• Isoelectric Point: 5.0074

>AT24828 WP_013209332.1 NC_014310:294503-294970 [Ralstonia solanacearum PSI07]
MTAIAPSRMTLPAEREVQAAVQGQRALAAYLATRVETQQIQIFDDQNQAHQVELPTSALRLLVDILAELADGNAVQVVPV
HAELTTQEAADLLNVSRPHLVKLLEEGELPFHRTGKHRRVRFADLMQFKEARDQASEQAIADLSRQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT24828 NC_014310:294503-294970 [Ralstonia solanacearum PSI07]
ATGACCGCCATCGCCCCATCCAGGATGACACTCCCCGCCGAGCGGGAAGTGCAGGCGGCCGTGCAAGGCCAACGGGCCTT
GGCGGCCTATCTGGCGACACGCGTCGAAACCCAACAGATTCAAATCTTTGATGATCAAAACCAGGCTCACCAGGTGGAGC
TGCCGACGTCTGCCTTGCGTCTATTGGTCGACATCCTGGCGGAACTCGCTGATGGCAATGCCGTCCAGGTGGTCCCGGTC
CATGCCGAGTTGACCACACAGGAGGCGGCGGATCTCTTGAATGTCTCCAGGCCGCACCTCGTCAAGTTGCTGGAAGAGGG
GGAGTTGCCCTTCCATCGGACGGGCAAGCATCGGCGAGTGCGCTTTGCTGACCTGATGCAGTTCAAGGAAGCGCGTGATC
AAGCCAGTGAGCAGGCGATCGCTGACCTGAGCCGTCAGGCGCAAGAGCTGGGTATGGGGTACGAATGA