Detailed information of TA system TA024825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3327864..3328483
• Replicon: chromosome
• Accession: NC_014306
• Organism: Erwinia billingiae Eb661

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D8MUK5
• Locus tag: EBC_RS16630
• Protein ID: WP_013202997.1
• Coordinates: 3328304..3328483 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D8MUK4
• Locus tag: EBC_RS16625
• Protein ID: WP_013202996.1
• Coordinates: 3327864..3328274 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBC_RS16605	3323205..3323897	+	693	WP_013202992.1	CidB/LrgB family autolysis modulator	-
EBC_RS16610	3324026..3324910	+	885	WP_013202993.1	cytidine deaminase	-
EBC_RS16615	3325077..3326774	+	1698	WP_013202994.1	NAD-dependent malic enzyme	-
EBC_RS16620	3326938..3327804	+	867	WP_013202995.1	outer membrane permeability protein SanA	-
EBC_RS16625	3327864..3328274	-	411	WP_013202996.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EBC_RS16630	3328304..3328483	-	180	WP_013202997.1	type II toxin-antitoxin system HicA family toxin	Toxin
EBC_RS16635	3328703..3329713	-	1011	WP_013202998.1	galactose/methyl galactoside ABC transporter permease MglC	-
EBC_RS16640	3329733..3331253	-	1521	WP_013202999.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
EBC_RS16645	3331350..3332345	-	996	WP_013203000.1	galactose/glucose ABC transporter substrate-binding protein MglB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6677.89 Da
• Isoelectric Point: 11.0775

>T24825 WP_013202997.1 NC_014306:c3328483-3328304 [Erwinia billingiae Eb661]
MDSRTLIAEIKADGWELIRVNGSHHHFRHPTKKGVVTVPHPKKDLPIKTIKSIKKQAGM

Nucleotide

• Length: 180 bp

>T24825 NC_014306:c3328483-3328304 [Erwinia billingiae Eb661]
ATGGATAGCAGGACGTTAATCGCTGAAATCAAAGCTGACGGTTGGGAACTGATTAGGGTAAATGGAAGTCATCATCATTT
CAGGCACCCTACCAAAAAAGGGGTAGTGACTGTCCCCCATCCAAAGAAAGATTTACCCATAAAAACGATAAAGAGTATTA
AGAAACAAGCGGGGATGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14559.55 Da
• Isoelectric Point: 4.3937

>AT24825 WP_013202996.1 NC_014306:c3328274-3327864 [Erwinia billingiae Eb661]
MLYPIAIENGDEHHAYGVTVPDLPGCFSAGETLEEAVNNAKEAITGHIELLIELGDDIPAVSSLSALVADSQYAGYTWAL
VDVDLARLLGGTEKINVTLPKSLIDRIDRCVATHPEFKSRSGFLAQVAIERIAKGV

Nucleotide

• Length: 411 bp

>AT24825 NC_014306:c3328274-3327864 [Erwinia billingiae Eb661]
ATGCTATATCCAATTGCTATTGAGAACGGTGACGAGCACCATGCTTACGGCGTGACCGTGCCAGATTTGCCCGGTTGTTT
TTCTGCCGGAGAAACGTTAGAAGAAGCCGTGAACAATGCCAAAGAGGCGATTACTGGACATATTGAATTGCTTATCGAGC
TGGGTGACGATATTCCGGCCGTATCCTCCCTGAGCGCTCTTGTTGCCGATAGTCAGTATGCTGGCTATACCTGGGCGCTT
GTCGATGTGGACCTGGCCCGGCTCTTAGGGGGCACTGAGAAAATCAACGTGACTTTACCGAAGTCACTTATTGACCGTAT
AGATCGCTGTGTGGCCACACACCCTGAGTTTAAAAGCCGTTCTGGTTTCCTTGCTCAGGTGGCGATTGAGCGTATTGCGA
AAGGCGTTTAA

Structures

Toxin

• AlphaFold DB: D8MUK5

Antitoxin

• AlphaFold DB: D8MUK4