Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5141127..5141752 Replicon chromosome
Accession NZ_CP099140
Organism Klebsiella pneumoniae strain RHB28-SO-C05

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NFK38_RS25045 Protein ID WP_002882817.1
Coordinates 5141127..5141510 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NFK38_RS25050 Protein ID WP_004150355.1
Coordinates 5141510..5141752 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK38_RS25030 (NFK38_25015) 5138493..5139395 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NFK38_RS25035 (NFK38_25020) 5139392..5140027 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NFK38_RS25040 (NFK38_25025) 5140024..5140953 + 930 WP_046654801.1 formate dehydrogenase accessory protein FdhE -
NFK38_RS25045 (NFK38_25030) 5141127..5141510 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NFK38_RS25050 (NFK38_25035) 5141510..5141752 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NFK38_RS25055 (NFK38_25040) 5141957..5142874 + 918 WP_004181612.1 alpha/beta hydrolase -
NFK38_RS25060 (NFK38_25045) 5142888..5143829 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NFK38_RS25065 (NFK38_25050) 5143874..5144311 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NFK38_RS25070 (NFK38_25055) 5144308..5145168 - 861 WP_046654802.1 virulence factor BrkB family protein -
NFK38_RS25075 (NFK38_25060) 5145162..5145761 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T248227 WP_002882817.1 NZ_CP099140:c5141510-5141127 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT248227 WP_004150355.1 NZ_CP099140:c5141752-5141510 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References