Detailed information of TA system TA024822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3123496..3124241
• Replicon: chromosome
• Accession: NC_014306
• Organism: Erwinia billingiae Eb661

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: D8MU26
• Locus tag: EBC_RS15730
• Protein ID: WP_013202818.1
• Coordinates: 3123496..3123987 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: D8MU27
• Locus tag: EBC_RS15735
• Protein ID: WP_013202819.1
• Coordinates: 3123975..3124241 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBC_RS15715	3119415..3120524	-	1110	WP_013202816.1	alkene reductase	-
EBC_RS15720	3120649..3121827	-	1179	WP_013202817.1	MFS transporter	-
EBC_RS15725	3122062..3123222	-	1161	WP_013199948.1	IS30 family transposase	-
EBC_RS15730	3123496..3123987	-	492	WP_013202818.1	GNAT family N-acetyltransferase	Toxin
EBC_RS15735	3123975..3124241	-	267	WP_013202819.1	DUF1778 domain-containing protein	Antitoxin
EBC_RS15740	3124446..3125516	-	1071	WP_013202820.1	iron-containing alcohol dehydrogenase family protein	-
EBC_RS15745	3125535..3126203	-	669	WP_041692053.1	ABC transporter permease	-
EBC_RS15750	3126191..3126979	-	789	WP_013202822.1	ATP-binding cassette domain-containing protein	-
EBC_RS15755	3126972..3127775	-	804	WP_013202823.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
EBC_RS15760	3127792..3128814	-	1023	WP_013202824.1	isopenicillin N synthase family oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3109757..3124241	14484
	-	flank	IS/Tn	-	-	3122062..3123222	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17471.25 Da
• Isoelectric Point: 9.3207

>T24822 WP_013202818.1 NC_014306:c3123987-3123496 [Erwinia billingiae Eb661]
VGKVTAPAPLNTTHILSEFHSGEAALDEWIRLRGLKNQSTGAARTFVVCREDSSHVVGFYSLATGSVTHAIAPGGLRRNM
PDPLPIIILARLAVGSSCQGQGLGADLLHDAILRICRVAENIGVRAIMVHALSDVAKQFYLHHGFQPSMTQGKTLFLRLP
TAI

Nucleotide

• Length: 492 bp

>T24822 NC_014306:c3123987-3123496 [Erwinia billingiae Eb661]
GTGGGAAAAGTAACCGCTCCAGCACCGCTAAATACGACTCACATTCTTTCAGAGTTTCACTCCGGGGAAGCTGCACTGGA
TGAATGGATCAGGCTCAGGGGGTTAAAGAACCAGTCGACCGGCGCCGCCAGAACGTTTGTGGTATGCCGGGAAGATTCTT
CACACGTGGTTGGTTTTTACTCTCTGGCCACCGGCAGTGTCACTCATGCCATTGCACCCGGTGGGCTCAGACGAAACATG
CCTGACCCCCTTCCCATCATAATTTTGGCCCGGCTGGCAGTTGGTTCGAGTTGCCAGGGCCAGGGTCTTGGTGCGGATTT
ACTGCACGATGCAATACTCCGTATTTGCCGGGTTGCGGAGAATATCGGCGTCCGGGCCATTATGGTGCATGCCTTGTCTG
ATGTGGCAAAACAGTTTTACCTGCATCATGGATTCCAGCCCTCAATGACACAGGGAAAAACACTTTTTCTCCGGTTACCT
ACTGCCATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10054.60 Da
• Isoelectric Point: 5.0898

>AT24822 WP_013202819.1 NC_014306:c3124241-3123975 [Erwinia billingiae Eb661]
MKSDVQLNIRAKEAQRVLIDTAAAILHKSRTDFILDIACQAAENVILDRRMFNLDDEQYAEFINMLDAPVKANPALDTLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT24822 NC_014306:c3124241-3123975 [Erwinia billingiae Eb661]
ATGAAATCTGATGTTCAGCTTAACATTCGGGCTAAAGAAGCCCAGCGCGTGTTGATCGATACCGCTGCCGCTATTCTTCA
TAAATCACGCACCGACTTTATACTAGACATCGCTTGCCAGGCTGCTGAAAACGTTATCCTTGATCGTCGGATGTTTAATC
TCGATGATGAACAGTACGCCGAGTTTATCAATATGCTGGATGCACCCGTTAAAGCGAATCCGGCACTGGATACGCTCCTG
GCGAGAAAACCGCAGTGGGAAAAGTAA

Structures

Toxin

• AlphaFold DB: D8MU26

Antitoxin

• AlphaFold DB: D8MU27