Detailed information of TA system TA024821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1201681..1202309
• Replicon: chromosome
• Accession: NC_014306
• Organism: Erwinia billingiae Eb661

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D8MP01
• Locus tag: EBC_RS06820
• Protein ID: WP_013201057.1
• Coordinates: 1201681..1201899 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D8MP02
• Locus tag: EBC_RS06825
• Protein ID: WP_013201058.1
• Coordinates: 1201929..1202309 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBC_RS06795	1197556..1197894	+	339	WP_007886947.1	P-II family nitrogen regulator	-
EBC_RS06800	1197930..1199219	+	1290	WP_041692221.1	ammonium transporter AmtB	-
EBC_RS06805	1199308..1200174	-	867	WP_013201054.1	acyl-CoA thioesterase II	-
EBC_RS06810	1200396..1200953	+	558	WP_013201055.1	YbaY family lipoprotein	-
EBC_RS06815	1200978..1201289	-	312	WP_013201056.1	MGMT family protein	-
EBC_RS06820	1201681..1201899	-	219	WP_013201057.1	hemolysin expression modulator Hha	Toxin
EBC_RS06825	1201929..1202309	-	381	WP_013201058.1	Hha toxicity modulator TomB	Antitoxin
EBC_RS06830	1202454..1202807	-	354	WP_013201059.1	hypothetical protein	-
EBC_RS06835	1203223..1203909	+	687	WP_013201060.1	ABC transporter ATP-binding protein	-
EBC_RS06840	1203912..1204778	+	867	WP_041692222.1	metal ABC transporter permease	-
EBC_RS06845	1204775..1205654	+	880	Protein_1068	metal ABC transporter substrate-binding protein	-
EBC_RS06850	1205747..1205887	-	141	WP_013201062.1	type B 50S ribosomal protein L36	-
EBC_RS06855	1205897..1206160	-	264	WP_013201063.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.10 Da
• Isoelectric Point: 9.4825

>T24821 WP_013201057.1 NC_014306:c1201899-1201681 [Erwinia billingiae Eb661]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T24821 NC_014306:c1201899-1201681 [Erwinia billingiae Eb661]
ATGACAGATAAACTTCTTACTAAAACTGATTATCTGATGCGTTTAAGACGGTGTCGGTCAATCGACACCCTCGAGCGCGT
AATCGAAAAAAACAAATACGAATTATCCGATAATGAACTGGCTGTGTTTTATTCTGCGGCTGATCATCGTCTGGCCGAAC
TGACGATGAATAAACTCTACGATAAAATCCCGGTAGCAGTTTGGAAGTTCGTTCGATAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14596.35 Da
• Isoelectric Point: 4.4699

>AT24821 WP_013201058.1 NC_014306:c1202309-1201929 [Erwinia billingiae Eb661]
MDEYSPKRHDIAQLKFVCENLYDESLATLGDSHHGWVNDPTAAVNLQLNDLIEHIAAFTMNYKIKHLEDSDLISQIDEYL
DDTFMLFSNYGINVQDLQRWQKSAKRLFNIFAEECALVPVQTSHSF

Nucleotide

• Length: 381 bp

>AT24821 NC_014306:c1202309-1201929 [Erwinia billingiae Eb661]
ATGGACGAATACTCACCAAAAAGGCATGATATCGCCCAGCTGAAATTTGTGTGTGAAAATTTGTACGATGAAAGCCTGGC
GACGTTAGGCGATAGTCATCACGGCTGGGTTAACGATCCTACTGCTGCAGTCAATTTACAGCTCAATGATCTGATTGAGC
ATATTGCTGCCTTCACCATGAATTACAAAATTAAACATCTCGAAGATAGTGACCTGATTAGTCAGATTGATGAATATCTC
GATGATACGTTTATGCTCTTTAGTAATTACGGCATTAATGTTCAGGATTTGCAGCGCTGGCAAAAATCAGCAAAACGCTT
GTTTAACATTTTCGCCGAGGAATGCGCTCTCGTGCCCGTACAGACGAGCCATTCATTTTAG

Structures

Toxin

• AlphaFold DB: D8MP01

Antitoxin

• AlphaFold DB: D8MP02