Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 108230..108811 Replicon plasmid pEB170
Accession NC_014305
Organism Erwinia billingiae Eb661

Toxin (Protein)


Gene name mazF Uniprot ID D8MK01
Locus tag EBC_RS24910 Protein ID WP_013199966.1
Coordinates 108479..108811 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A328T8X3
Locus tag EBC_RS01035 Protein ID WP_041691825.1
Coordinates 108230..108478 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBC_RS01020 104793..105812 - 1020 WP_013199962.1 replication initiation protein -
EBC_RS25660 106865..107798 + 934 Protein_91 transposase -
EBC_RS01035 108230..108478 + 249 WP_041691825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EBC_RS24910 108479..108811 + 333 WP_013199966.1 endoribonuclease MazF Toxin
EBC_RS24915 109424..109651 - 228 WP_071822088.1 DNA partition complex ParG -
EBC_RS01045 109690..110310 - 621 WP_013199968.1 ParA family protein -
EBC_RS01050 110839..112113 - 1275 WP_013199970.1 translesion error-prone DNA polymerase V subunit UmuC -
EBC_RS01055 112117..112512 - 396 WP_041691826.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
EBC_RS24920 112663..112875 + 213 WP_013199972.1 hypothetical protein -
EBC_RS01060 113165..113362 - 198 WP_013199974.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..169778 169778


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

Antitoxin PemI-like

(4-81)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11955.70 Da        Isoelectric Point: 7.1057

>T24820 WP_013199966.1 NC_014305:108479-108811 [Erwinia billingiae Eb661]
MVSRYVPNSGDLIWLDFDPVEGHEQGGHRPAVVLSPFSYNNPVGLLLCVPCTTKAKGYPFEVELSGSRESVALADQVTCV
DWRARKVTKKGTVSPDELAEIRAKAKALIG

Download         Length: 333 bp

>T24820 NC_014305:108479-108811 [Erwinia billingiae Eb661]
ATGGTTTCACGTTACGTACCAAATTCTGGTGATCTAATCTGGCTGGATTTTGACCCCGTAGAAGGTCACGAACAGGGCGG
CCATCGCCCTGCTGTCGTCCTTAGCCCTTTTTCTTACAATAATCCCGTAGGATTGCTGCTTTGCGTTCCGTGTACCACTA
AAGCTAAAGGCTATCCATTTGAGGTAGAACTATCCGGTAGCCGCGAAAGTGTTGCTCTTGCCGATCAAGTTACCTGTGTT
GACTGGAGAGCGCGAAAAGTAACAAAAAAAGGCACTGTATCGCCTGACGAACTTGCTGAGATCAGAGCAAAGGCTAAAGC
TCTAATTGGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8815.28 Da        Isoelectric Point: 5.7630

>AT24820 WP_041691825.1 NC_014305:108230-108478 [Erwinia billingiae Eb661]
MTTVTLKKWGNSPSIRIPASVMQAAALNVEDKLDLKIDEAGQIVLVPLKSKEYSLDVLLSGITPDNVHGKIDFGSPVGKE
LI

Download         Length: 249 bp

>AT24820 NC_014305:108230-108478 [Erwinia billingiae Eb661]
ATGACAACAGTAACGCTAAAAAAATGGGGCAATAGCCCATCGATCAGAATACCAGCCAGCGTAATGCAAGCAGCAGCACT
TAATGTCGAAGATAAACTCGATTTGAAAATTGATGAAGCTGGCCAAATTGTTTTAGTACCATTGAAATCTAAAGAGTATT
CACTAGATGTACTGTTATCAGGTATCACACCAGATAACGTTCACGGAAAAATAGATTTTGGTTCGCCAGTTGGAAAGGAA
TTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D8MK01

Antitoxin

Source ID Structure
AlphaFold DB A0A328T8X3

References