Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2259908..2260434 Replicon chromosome
Accession NZ_CP099084
Organism Citrobacter freundii strain RHB43-C17

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NFK79_RS11060 Protein ID WP_000323025.1
Coordinates 2260147..2260434 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NFK79_RS11055 Protein ID WP_000534858.1
Coordinates 2259908..2260147 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK79_RS11030 (2254908) 2254908..2255789 + 882 WP_279264806.1 dsDNA nuclease domain-containing protein -
NFK79_RS11035 (2255789) 2255789..2257441 + 1653 WP_001575467.1 hypothetical protein -
NFK79_RS11040 (2258038) 2258038..2258478 + 441 WP_279264781.1 hypothetical protein -
NFK79_RS11045 (2258754) 2258754..2259506 - 753 WP_279264782.1 hypothetical protein -
NFK79_RS11050 (2259779) 2259779..2259883 - 105 Protein_2161 protein YdfV -
NFK79_RS11055 (2259908) 2259908..2260147 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NFK79_RS11060 (2260147) 2260147..2260434 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NFK79_RS11065 (2260491) 2260491..2261414 + 924 Protein_2164 aldehyde dehydrogenase family protein -
NFK79_RS11070 (2261426) 2261426..2261899 + 474 WP_181499357.1 ester cyclase -
NFK79_RS11075 (2261982) 2261982..2263874 + 1893 WP_181499358.1 NAD(P)/FAD-dependent oxidoreductase -
NFK79_RS11080 (2263878) 2263878..2265089 + 1212 WP_096757185.1 cytochrome c -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2246342..2260434 14092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T248189 WP_000323025.1 NZ_CP099084:2260147-2260434 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT248189 WP_000534858.1 NZ_CP099084:2259908-2260147 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References