Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4851012..4851628 Replicon chromosome
Accession NZ_CP099037
Organism Citrobacter freundii strain RHB44-SO-C05

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag NFL15_RS23390 Protein ID WP_003028682.1
Coordinates 4851012..4851386 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag NFL15_RS23395 Protein ID WP_043018956.1
Coordinates 4851386..4851628 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFL15_RS23375 (4848515) 4848515..4849417 + 903 WP_016155183.1 formate dehydrogenase O subunit beta -
NFL15_RS23380 (4849414) 4849414..4850049 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFL15_RS23385 (4850046) 4850046..4850975 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFL15_RS23390 (4851012) 4851012..4851386 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
NFL15_RS23395 (4851386) 4851386..4851628 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
NFL15_RS23400 (4851834) 4851834..4852742 + 909 WP_032950680.1 alpha/beta hydrolase -
NFL15_RS23405 (4852893) 4852893..4853834 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NFL15_RS23410 (4853879) 4853879..4854316 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
NFL15_RS23415 (4854313) 4854313..4855185 - 873 WP_003028669.1 virulence factor BrkB family protein -
NFL15_RS23420 (4855179) 4855179..4855778 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T248183 WP_003028682.1 NZ_CP099037:c4851386-4851012 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT248183 WP_043018956.1 NZ_CP099037:c4851628-4851386 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References