Detailed information of TA system TA248165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 141249..142872
• Replicon: plasmid pYUGDPS1-1
• Accession: NZ_CP098835
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain GD19PS1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: NFH28_RS25140
• Protein ID: WP_000088046.1
• Coordinates: 141249..142574 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: NFH28_RS25145
• Protein ID: WP_024136327.1
• Coordinates: 142579..142872 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NFH28_RS25110 (NFH28_25110)	137919..138218	-	300	WP_001572440.1	hypothetical protein	-
NFH28_RS25115 (NFH28_25115)	138273..138509	-	237	Protein_153	type I restriction-modification system subunit M N-terminal domain-containing protein	-
NFH28_RS25120 (NFH28_25120)	138963..139388	-	426	WP_001043843.1	hypothetical protein	-
NFH28_RS25125 (NFH28_25125)	139642..140457	-	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
NFH28_RS25130 (NFH28_25130)	140470..140880	-	411	WP_000985911.1	hypothetical protein	-
NFH28_RS25135 (NFH28_25135)	140982..141188	-	207	WP_000134171.1	hypothetical protein	-
NFH28_RS25140 (NFH28_25140)	141249..142574	-	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
NFH28_RS25145 (NFH28_25145)	142579..142872	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
NFH28_RS25150 (NFH28_25150)	143476..143868	-	393	WP_000341066.1	hypothetical protein	-
NFH28_RS25155 (NFH28_25155)	144241..144582	+	342	WP_001151575.1	hypothetical protein	-
NFH28_RS25160 (NFH28_25160)	144674..144865	+	192	WP_000398480.1	hypothetical protein	-
NFH28_RS25165 (NFH28_25165)	144915..145196	+	282	WP_000077926.1	hypothetical protein	-
NFH28_RS25890	145537..145668	-	132	WP_000374059.1	hypothetical protein	-
NFH28_RS25170 (NFH28_25170)	145759..147000	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1	-	1..241147	241147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T248165 WP_000088046.1 NZ_CP098835:c142574-141249 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT248165 WP_024136327.1 NZ_CP098835:c142872-142579 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1