Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-Xre
Location 407454..408334 Replicon plasmid pA
Accession NZ_CP098808
Organism Ensifer adhaerens strain M8

Toxin (Protein)


Gene name - Uniprot ID A0A1G9YN53
Locus tag NE863_RS21180 Protein ID WP_060529103.1
Coordinates 407825..408334 (+) Length 170 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1G9YN41
Locus tag NE863_RS21175 Protein ID WP_060529162.1
Coordinates 407454..407825 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NE863_RS21155 (NE863_21155) 403347..403730 + 384 WP_060529107.1 RidA family protein -
NE863_RS21160 (NE863_21160) 403746..405734 + 1989 WP_060529106.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
NE863_RS21165 (NE863_21165) 405731..406600 + 870 WP_060529105.1 hydroxymethylglutaryl-CoA lyase -
NE863_RS21170 (NE863_21170) 406597..407382 + 786 WP_192357865.1 crotonase/enoyl-CoA hydratase family protein -
NE863_RS21175 (NE863_21175) 407454..407825 + 372 WP_060529162.1 DUF2384 domain-containing protein Antitoxin
NE863_RS21180 (NE863_21180) 407825..408334 + 510 WP_060529103.1 RES domain-containing protein Toxin
NE863_RS21185 (NE863_21185) 408367..409731 - 1365 WP_060529102.1 FAD-binding oxidoreductase -
NE863_RS21190 (NE863_21190) 409895..411019 - 1125 WP_252160832.1 sulfate/molybdate ABC transporter ATP-binding protein -
NE863_RS21195 (NE863_21195) 411050..411970 - 921 WP_112773461.1 sulfate ABC transporter permease subunit CysW -
NE863_RS21200 (NE863_21200) 411960..412826 - 867 WP_082936543.1 sulfate ABC transporter permease subunit CysT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - katA 1..1753114 1753114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(8-162)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(71-120)


Sequences

Toxin        


Download         Length: 170 a.a.        Molecular weight: 18727.30 Da        Isoelectric Point: 6.3310

>T248109 WP_060529103.1 NZ_CP098808:407825-408334 [Ensifer adhaerens]
MAVGAPVRLWRAYVPRWAHAPLSGEGAGRYGGRWNPVGTPTIYAARELSTAWAEYNQGFVQHPALIAQLELRFARLADLT
APEILSDLGLTEEIHRCEWRDALDRGLVPETHRLQLALSDRGFDGLIYPSFMSPGGTCVALWHWNAGEGSELKVIDPEGR
LPKTPASWL

Download         Length: 510 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 12900.86 Da        Isoelectric Point: 9.8060

>AT248109 WP_060529162.1 NZ_CP098808:407454-407825 [Ensifer adhaerens]
MAAVGLNISTAHYGESHSSFLSARLVADRLGVTLAELAKLIGVARNTLTAKSSARKVDAALSSVVRILAMAGEMAGDDSR
AVIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLEAVRSGVYA

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G9YN53

Antitoxin

Source ID Structure
AlphaFold DB A0A1G9YN41

References