Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93049..94672 Replicon plasmid pYQ13422-1
Accession NZ_CP098779
Organism Enterobacter hormaechei strain YQ13422hy

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag ND013_RS22265 Protein ID WP_000088044.1
Coordinates 93347..94672 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag ND013_RS22260 Protein ID WP_012477377.1
Coordinates 93049..93342 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND013_RS22240 88848..89240 + 393 WP_000341066.1 hypothetical protein -
ND013_RS22245 89899..90555 + 657 WP_000462754.1 trypsin-like serine protease -
ND013_RS22250 90758..91255 + 498 WP_000340139.1 membrane protein -
ND013_RS22255 91260..92648 + 1389 WP_098992326.1 hypothetical protein -
ND013_RS22260 93049..93342 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
ND013_RS22265 93347..94672 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
ND013_RS22270 94733..94939 + 207 WP_000134171.1 hypothetical protein -
ND013_RS22275 95040..95450 + 411 WP_000985909.1 hypothetical protein -
ND013_RS22280 95463..96278 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
ND013_RS22285 96531..96956 + 426 WP_000278471.1 hypothetical protein -
ND013_RS22290 97505..97813 + 309 WP_001224686.1 hypothetical protein -
ND013_RS22295 97829..98146 + 318 WP_012477379.1 hypothetical protein -
ND013_RS22300 98328..99332 - 1005 WP_000427619.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / aac(3)-IId / blaTEM-1B / blaSFO-1 / catA2 / tet(D) / blaSHV-12 / sul1 / blaDHA-1 / qnrB4 / qacE / ere(A) / aac(6')-IIc - 1..295136 295136
- flank IS/Tn - - 98328..99332 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T248088 WP_000088044.1 NZ_CP098779:93347-94672 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT248088 WP_012477377.1 NZ_CP098779:93049-93342 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References