Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpA-brnA/BrnT-BrnA
Location 54879..55464 Replicon plasmid unnamed2
Accession NZ_CP098764
Organism Sphingomonas aerolata strain PDD-32b-11

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag NEF64_RS18705 Protein ID WP_159513481.1
Coordinates 55138..55464 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID -
Locus tag NEF64_RS18700 Protein ID WP_082446612.1
Coordinates 54879..55157 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEF64_RS18675 (NEF64_18675) 49968..50192 - 225 WP_133192513.1 conjugal transfer protein TraD -
NEF64_RS18680 (NEF64_18680) 50206..50535 - 330 WP_252574998.1 conjugal transfer protein TraD -
NEF64_RS18685 (NEF64_18685) 50710..53625 + 2916 WP_252575000.1 Ti-type conjugative transfer relaxase TraA -
NEF64_RS18690 (NEF64_18690) 53629..54366 + 738 WP_156360267.1 DUF6118 family protein -
NEF64_RS18695 (NEF64_18695) 54402..54746 + 345 WP_156360266.1 hypothetical protein -
NEF64_RS18700 (NEF64_18700) 54879..55157 - 279 WP_082446612.1 BrnA antitoxin family protein Antitoxin
NEF64_RS18705 (NEF64_18705) 55138..55464 - 327 WP_159513481.1 BrnT family toxin Toxin
NEF64_RS18710 (NEF64_18710) 55528..56025 - 498 WP_235516303.1 hypothetical protein -
NEF64_RS18715 (NEF64_18715) 56069..56632 - 564 WP_252575002.1 recombinase family protein -
NEF64_RS18720 (NEF64_18720) 56819..57001 + 183 WP_252575005.1 hypothetical protein -
NEF64_RS18725 (NEF64_18725) 57078..57410 + 333 WP_252575082.1 YnfA family protein -
NEF64_RS18730 (NEF64_18730) 57622..57978 + 357 Protein_49 recombinase family protein -
NEF64_RS18735 (NEF64_18735) 58268..59434 + 1167 WP_252575012.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..77112 77112
- flank IS/Tn - - 56069..56632 563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(18-99)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-87)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12174.80 Da        Isoelectric Point: 6.0798

>T248026 WP_159513481.1 NZ_CP098764:c55464-55138 [Sphingomonas aerolata]
MGCTYNIVNAGVGVSFEWDDRKAVFNLAKHGVSFELAKEVWNDPLHVIIPDRVEGAEQRWHALGLVGPIVVLVVVHSYPD
AGDEDRIRIISARKATKQERYRYEQEGA

Download         Length: 327 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10494.90 Da        Isoelectric Point: 6.4645

>AT248026 WP_082446612.1 NZ_CP098764:c55157-54879 [Sphingomonas aerolata]
MSKKALSPELQDQLNNLAAMPDDQIDTNDIPEALPEAWLHARQPGLYRPIKKPVTLRLDADVVAWFKEHAQGRGYQTDIN
RVLRRYVSEAQA

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References