247981

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 60695..61832 Replicon plasmid pEFM9-1
Accession NZ_CP098744
Organism Enterococcus faecalis strain M9

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag NDO76_RS13455 Protein ID WP_002332783.1
Coordinates 60695..61558 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag NDO76_RS13460 Protein ID WP_002326825.1
Coordinates 61560..61832 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NDO76_RS13425 (NDO76_13425) 56082..56876 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
NDO76_RS14085 57286..57369 + 84 Protein_69 MLS leader peptide -
NDO76_RS13430 (NDO76_13430) 57418..57501 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
NDO76_RS13435 (NDO76_13435) 57626..58393 + 768 WP_172688991.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
NDO76_RS13440 (NDO76_13440) 58421..59101 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
NDO76_RS13445 (NDO76_13445) 59135..59641 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
NDO76_RS13450 (NDO76_13450) 59938..60255 - 318 WP_002326830.1 hypothetical protein -
NDO76_RS13455 (NDO76_13455) 60695..61558 - 864 WP_002332783.1 zeta toxin family protein Toxin
NDO76_RS13460 (NDO76_13460) 61560..61832 - 273 WP_002326825.1 antitoxin Antitoxin
NDO76_RS13465 (NDO76_13465) 61850..62065 - 216 WP_001835296.1 peptide-binding protein -
NDO76_RS13470 (NDO76_13470) 62157..63053 - 897 WP_002326827.1 ParA family protein -
NDO76_RS13475 (NDO76_13475) 63156..63416 - 261 Protein_79 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
NDO76_RS13480 (NDO76_13480) 63586..64323 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
NDO76_RS13485 (NDO76_13485) 64448..64531 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
NDO76_RS14090 64580..64663 - 84 Protein_82 MLS leader peptide -
NDO76_RS13490 (NDO76_13490) 64963..65334 - 372 WP_002358205.1 hypothetical protein -
NDO76_RS13495 (NDO76_13495) 65327..66172 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cat / tet(L) / tet(M) / erm(A) / optrA / fexA / ant(6)-Ia / aph(3')-III / erm(B) / dfrG - 1..66643 66643
- inside IScluster/Tn cat / tet(L) / tet(M) / erm(A) / optrA / fexA / ant(6)-Ia / aph(3')-III / erm(B) / dfrG - 24451..64323 39872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T247981 WP_002332783.1 NZ_CP098744:c61558-60695 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT247981 WP_002326825.1 NZ_CP098744:c61832-61560 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References