Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
| Location | 9162..9733 | Replicon | plasmid pEFM9-1 |
| Accession | NZ_CP098744 | ||
| Organism | Enterococcus faecalis strain M9 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A2Z2CGM4 |
| Locus tag | NDO76_RS13180 | Protein ID | WP_002362432.1 |
| Coordinates | 9392..9733 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | R3KHK9 |
| Locus tag | NDO76_RS13175 | Protein ID | WP_002362431.1 |
| Coordinates | 9162..9392 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NDO76_RS13120 (NDO76_13120) | 4722..5018 | - | 297 | WP_002367795.1 | hypothetical protein | - |
| NDO76_RS13125 (NDO76_13125) | 5275..5361 | - | 87 | WP_012881081.1 | type I toxin-antitoxin system Fst family toxin | - |
| NDO76_RS13130 (NDO76_13130) | 5453..5668 | - | 216 | WP_002415356.1 | hypothetical protein | - |
| NDO76_RS13135 (NDO76_13135) | 5616..6134 | - | 519 | WP_002367793.1 | hypothetical protein | - |
| NDO76_RS13140 (NDO76_13140) | 6550..6891 | - | 342 | WP_002367792.1 | bacteriocin immunity protein | - |
| NDO76_RS13145 (NDO76_13145) | 6892..7107 | - | 216 | WP_002367791.1 | leucocin A/sakacin P family class II bacteriocin | - |
| NDO76_RS13150 (NDO76_13150) | 7277..7468 | - | 192 | WP_002367788.1 | hypothetical protein | - |
| NDO76_RS13155 (NDO76_13155) | 7480..7674 | - | 195 | WP_002367787.1 | hypothetical protein | - |
| NDO76_RS13160 (NDO76_13160) | 7834..8040 | - | 207 | WP_002367786.1 | hypothetical protein | - |
| NDO76_RS13165 (NDO76_13165) | 8034..8348 | - | 315 | WP_002367785.1 | hypothetical protein | - |
| NDO76_RS13170 (NDO76_13170) | 8338..8958 | - | 621 | WP_002367784.1 | recombinase family protein | - |
| NDO76_RS13175 (NDO76_13175) | 9162..9392 | + | 231 | WP_002362431.1 | hypothetical protein | Antitoxin |
| NDO76_RS13180 (NDO76_13180) | 9392..9733 | + | 342 | WP_002362432.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| NDO76_RS13185 (NDO76_13185) | 9845..10783 | + | 939 | WP_251846814.1 | hypothetical protein | - |
| NDO76_RS13190 (NDO76_13190) | 11051..11653 | + | 603 | WP_002367780.1 | Fic family protein | - |
| NDO76_RS13195 (NDO76_13195) | 11887..12567 | - | 681 | WP_129217752.1 | IS6 family transposase | - |
| NDO76_RS13200 (NDO76_13200) | 12822..13760 | + | 939 | WP_002335374.1 | 2-dehydropantoate 2-reductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | cat / tet(L) / tet(M) / erm(A) / optrA / fexA / ant(6)-Ia / aph(3')-III / erm(B) / dfrG | - | 1..66643 | 66643 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13244.44 Da Isoelectric Point: 8.0113
>T247980 WP_002362432.1 NZ_CP098744:9392-9733 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T247980 NZ_CP098744:9392-9733 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8510.42 Da Isoelectric Point: 4.3855
>AT247980 WP_002362431.1 NZ_CP098744:9162-9392 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
Download Length: 231 bp
>AT247980 NZ_CP098744:9162-9392 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z2CGM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3KHK9 |