Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 522516..523152 Replicon chromosome
Accession NZ_CP098738
Organism Bacillus halotolerans strain XE48

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NDR85_RS02665 Protein ID WP_003156187.1
Coordinates 522802..523152 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NDR85_RS02660 Protein ID WP_003225183.1
Coordinates 522516..522797 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NDR85_RS02640 (NDR85_02640) 518873..519472 - 600 WP_263870885.1 rhomboid family intramembrane serine protease -
NDR85_RS02645 (NDR85_02645) 519567..519932 + 366 WP_044161735.1 holo-ACP synthase -
NDR85_RS02650 (NDR85_02650) 520098..521114 + 1017 WP_256766957.1 outer membrane lipoprotein carrier protein LolA -
NDR85_RS02655 (NDR85_02655) 521231..522400 + 1170 WP_044161733.1 alanine racemase -
NDR85_RS02660 (NDR85_02660) 522516..522797 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NDR85_RS02665 (NDR85_02665) 522802..523152 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NDR85_RS02670 (NDR85_02670) 523268..524092 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
NDR85_RS02675 (NDR85_02675) 524097..524462 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NDR85_RS02680 (NDR85_02680) 524466..524867 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
NDR85_RS02685 (NDR85_02685) 524879..525886 + 1008 WP_044161721.1 phosphoserine phosphatase RsbU -
NDR85_RS02690 (NDR85_02690) 525947..526276 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
NDR85_RS02695 (NDR85_02695) 526273..526755 + 483 WP_024120302.1 anti-sigma B factor RsbW -
NDR85_RS02700 (NDR85_02700) 526721..527509 + 789 WP_044161720.1 RNA polymerase sigma factor SigB -
NDR85_RS02705 (NDR85_02705) 527509..528108 + 600 WP_044161719.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T247942 WP_003156187.1 NZ_CP098738:522802-523152 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT247942 WP_003225183.1 NZ_CP098738:522516-522797 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References