Detailed information of TA system TA024784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2864682..2865251
• Replicon: chromosome
• Accession: NC_014219
• Organism: Bacillus selenitireducens MLS10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D6XXU1
• Locus tag: BSEL_RS13355
• Protein ID: WP_013173554.1
• Coordinates: 2864919..2865251 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6XXU0
• Locus tag: BSEL_RS13350
• Protein ID: WP_013173553.1
• Coordinates: 2864682..2864918 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSEL_RS13335 (Bsel_2628)	2861858..2862469	+	612	WP_013173549.1	TetR/AcrR family transcriptional regulator	-
BSEL_RS13340 (Bsel_2630)	2862719..2863837	-	1119	WP_013173551.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-
BSEL_RS18235 (Bsel_2631)	2863907..2864041	+	135	WP_013171562.1	hypothetical protein	-
BSEL_RS13345 (Bsel_2632)	2864087..2864320	-	234	WP_013173552.1	hypothetical protein	-
BSEL_RS13350 (Bsel_2633)	2864682..2864918	+	237	WP_013173553.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSEL_RS13355 (Bsel_2634)	2864919..2865251	+	333	WP_013173554.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BSEL_RS13360 (Bsel_2635)	2865355..2866086	-	732	WP_013173555.1	7-carboxy-7-deazaguanine synthase QueE	-
BSEL_RS13365 (Bsel_2636)	2866079..2866561	-	483	WP_013173556.1	6-carboxytetrahydropterin synthase QueD	-
BSEL_RS13370 (Bsel_2637)	2866562..2867230	-	669	WP_177304838.1	7-cyano-7-deazaguanine synthase QueC	-
BSEL_RS13375 (Bsel_2638)	2867441..2868559	-	1119	WP_013173558.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-
BSEL_RS17885 (Bsel_2639)	2868623..2868763	+	141	WP_013173559.1	hypothetical protein	-
BSEL_RS13380 (Bsel_2640)	2869102..2870157	+	1056	WP_013173560.1	extracellular solute-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2857503..2870157	12654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12070.73 Da
• Isoelectric Point: 4.8000

>T24784 WP_013173554.1 NC_014219:2864919-2865251 [[Bacillus] selenitireducens MLS10]
MSEVADRGNLVYLNFNPQAGHEQTGSRSAIVISPESFNRITGFAVVCPITSVSKDYPFEVALPDGLAIHGVILTDQIKSL
DWKARKLNVVDEAPAETVQECLEKIHTFIT

Nucleotide

• Length: 333 bp

>T24784 NC_014219:2864919-2865251 [[Bacillus] selenitireducens MLS10]
ATGTCGGAAGTTGCTGACCGTGGAAATCTTGTTTATTTGAATTTCAACCCACAAGCAGGACACGAACAAACGGGTTCAAG
ATCTGCTATAGTTATTTCACCCGAATCTTTCAATAGAATCACAGGTTTTGCCGTAGTATGTCCGATTACCAGTGTTTCTA
AGGATTATCCCTTTGAGGTAGCCTTACCGGATGGTTTGGCTATCCACGGGGTTATCTTAACCGATCAGATAAAAAGTCTG
GATTGGAAAGCAAGAAAGCTCAATGTGGTTGATGAAGCACCTGCTGAAACCGTACAAGAATGTTTGGAGAAGATACATAC
ATTCATTACTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8720.96 Da
• Isoelectric Point: 6.7149

>AT24784 WP_013173553.1 NC_014219:2864682-2864918 [[Bacillus] selenitireducens MLS10]
MTTKLQKWGNSLGVRIPKSHVQQLNVSDGSEIEITLINNSIVIKPVTKKPTLDELLSQVTEDNKHTEIDCHSTGRELL

Nucleotide

• Length: 237 bp

>AT24784 NC_014219:2864682-2864918 [[Bacillus] selenitireducens MLS10]
ATGACGACTAAGCTTCAAAAATGGGGTAACAGTCTCGGTGTAAGAATCCCTAAGTCTCATGTCCAACAACTTAACGTTTC
AGATGGTAGTGAAATTGAAATCACTCTCATTAATAATTCCATTGTGATAAAACCAGTGACTAAGAAACCGACATTGGACG
AATTATTATCCCAAGTAACTGAAGATAATAAGCACACTGAAATTGACTGTCATTCAACGGGAAGGGAGTTACTTTAA

Structures

Toxin

• AlphaFold DB: D6XXU1

Antitoxin

• AlphaFold DB: D6XXU0