Detailed information of TA system TA024783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 564592..565224
• Replicon: chromosome
• Accession: NC_014219
• Organism: Bacillus selenitireducens MLS10

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BSEL_RS02790
• Protein ID: WP_041581698.1
• Coordinates: 564874..565224 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6XXZ4
• Locus tag: BSEL_RS02785
• Protein ID: WP_013171496.1
• Coordinates: 564592..564870 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSEL_RS02765 (Bsel_0527)	560368..561135	-	768	WP_013171492.1	rhomboid family intramembrane serine protease	-
BSEL_RS02770 (Bsel_0528)	561239..561634	+	396	WP_013171493.1	holo-ACP synthase	-
BSEL_RS02775 (Bsel_0529)	561637..563208	+	1572	WP_013171494.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
BSEL_RS02780 (Bsel_0530)	563205..564422	+	1218	WP_013171495.1	alanine racemase	-
BSEL_RS02785 (Bsel_0531)	564592..564870	+	279	WP_013171496.1	hypothetical protein	Antitoxin
BSEL_RS02790 (Bsel_0532)	564874..565224	+	351	WP_041581698.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BSEL_RS02795 (Bsel_0533)	565403..566263	+	861	WP_013171498.1	RsbT co-antagonist protein RsbRA	-
BSEL_RS02800 (Bsel_0534)	566244..566600	+	357	WP_013171499.1	STAS domain-containing protein	-
BSEL_RS02805 (Bsel_0535)	566605..567006	+	402	WP_013171500.1	anti-sigma regulatory factor	-
BSEL_RS02810 (Bsel_0536)	567033..568046	+	1014	WP_013171501.1	PP2C family protein-serine/threonine phosphatase	-
BSEL_RS02815 (Bsel_0537)	568111..568440	+	330	WP_013171502.1	STAS domain-containing protein	-
BSEL_RS02820 (Bsel_0538)	568440..568937	+	498	WP_013171503.1	anti-sigma B factor RsbW	-
BSEL_RS02825 (Bsel_0539)	568972..569706	+	735	WP_232970505.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12874.01 Da
• Isoelectric Point: 8.6233

>T24783 WP_041581698.1 NC_014219:564874-565224 [[Bacillus] selenitireducens MLS10]
MMVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKNGFDRDSVILLEQI
RTIDKQRLTDKITHLDEKTMAKVDEALRVSIGLIHL

Nucleotide

• Length: 351 bp

>T24783 NC_014219:564874-565224 [[Bacillus] selenitireducens MLS10]
TTGATGGTCAAACGAGGCGATGTTTACTTCGCTGATCTGTCACCCGTGGTCGGATCGGAACAGGGTGGCGTCCGTCCGGT
GCTCATTATTCAAAATGACATCGGAAACAGATTCAGCCCGACTGTGATTGTCGCTGCGATAACAGCGCAAATCCAGAAGG
CGAAGTTACCAACACATGTTGAAATCGATGCAAAAAAAAATGGGTTTGATCGTGATTCCGTCATTTTGCTTGAACAGATT
CGTACGATTGACAAGCAAAGACTGACAGATAAGATCACTCATCTTGACGAAAAGACGATGGCGAAAGTTGACGAAGCGCT
TCGTGTCAGTATCGGACTTATTCATTTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10543.98 Da
• Isoelectric Point: 4.5177

>AT24783 WP_013171496.1 NC_014219:564592-564870 [[Bacillus] selenitireducens MLS10]
VSADCTKKISVNLPQHLVHEIDALTEGEGCNRDELIHRELMRFVEIQRTKQIREEMERGYMEMAKINLNIASESFLVEEE
AESALDRLVSGV

Nucleotide

• Length: 279 bp

>AT24783 NC_014219:564592-564870 [[Bacillus] selenitireducens MLS10]
GTGTCAGCAGACTGCACGAAGAAAATCAGCGTAAACTTGCCGCAGCATCTGGTTCACGAGATTGATGCATTAACAGAAGG
TGAAGGATGTAACCGGGATGAACTGATTCACCGGGAATTGATGCGCTTTGTTGAAATACAGCGCACCAAGCAGATTCGTG
AAGAAATGGAACGCGGGTACATGGAAATGGCAAAGATCAACCTGAACATTGCGTCAGAATCGTTTTTGGTTGAAGAGGAG
GCCGAAAGTGCGCTCGACCGGCTGGTCAGCGGGGTGTAA

Structures

Antitoxin

• AlphaFold DB: D6XXZ4