Detailed information of TA system TA247815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3192473..3194193
• Replicon: chromosome
• Accession: NZ_CP098701
• Organism: Yersinia ruckeri strain NVI-5089

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: ND440_RS14715
• Protein ID: WP_096823271.1
• Coordinates: 3193537..3194193 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: ND440_RS14710
• Protein ID: WP_096823272.1
• Coordinates: 3192473..3193540 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND440_RS14660 (ND440_14655)	3188719..3188892	-	174	Protein_2840	type IV toxin-antitoxin system YeeU family antitoxin	-
ND440_RS14665 (ND440_14660)	3188918..3189043	-	126	Protein_2841	antirestriction protein	-
ND440_RS14670 (ND440_14665)	3189411..3190361	-	951	WP_038275283.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ND440_RS14675 (ND440_14670)	3190354..3190749	-	396	WP_038245317.1	DUF6088 family protein	-
ND440_RS14680 (ND440_14675)	3190824..3191054	-	231	Protein_2844	DUF4942 domain-containing protein	-
ND440_RS14685 (ND440_14680)	3191204..3191332	+	129	Protein_2845	helix-turn-helix domain-containing protein	-
ND440_RS14690 (ND440_14685)	3191325..3191445	+	121	Protein_2846	transposase	-
ND440_RS14695 (ND440_14690)	3191474..3191770	-	297	Protein_2847	integrase core domain-containing protein	-
ND440_RS14700 (ND440_14695)	3191769..3192167	+	399	Protein_2848	integrase core domain-containing protein	-
ND440_RS14705 (ND440_14700)	3192186..3192452	-	267	Protein_2849	hypothetical protein	-
ND440_RS14710 (ND440_14705)	3192473..3193540	-	1068	WP_096823272.1	macro domain-containing protein	Antitoxin
ND440_RS14715 (ND440_14710)	3193537..3194193	-	657	WP_096823271.1	DUF4433 domain-containing protein	Toxin
ND440_RS14720 (ND440_14715)	3194196..3194771	-	576	WP_234049239.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
ND440_RS14725 (ND440_14720)	3194895..3195284	-	390	WP_234049240.1	restriction endonuclease subunit S	-
ND440_RS14730 (ND440_14725)	3195548..3195838	-	291	WP_038275268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ND440_RS14735 (ND440_14730)	3195828..3196082	-	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ND440_RS14745 (ND440_14740)	3197428..3197673	+	246	Protein_2857	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3186962..3201094	14132
	-	inside	IScluster/Tn	-	-	3191474..3197377	5903

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25214.72 Da
• Isoelectric Point: 6.6007

>T247815 WP_096823271.1 NZ_CP098701:c3194193-3193537 [Yersinia ruckeri]
VAYDYSTSLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAEDWINIGNPELIDKRAGHLVPTGMRGTLHDYVPFYFTP
FSPMLMNIRSGRGGIKQRPNEEIVILVSSLRNMVAQNVSFVFTDSHAYYNLANYFTELDSLNQIDWPILQARDFRRDQDD
LAKFERYQAEALIWKHCPIALLGGMICYNDGVRLQLEQWLSQRNLSMPVHARAGWYFS

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40847.21 Da
• Isoelectric Point: 6.3136

>AT247815 WP_096823272.1 NZ_CP098701:c3193540-3192473 [Yersinia ruckeri]
MITFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPVNMKAYALACKHKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWVEDGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWSDVRMRIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARALIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDTQPLDVIWFNDQEKEHINLWLNNEAKEWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGKCQPTLDSVKEG
LHQWPAGERWASRKMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp