Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1967424..1968022 Replicon chromosome
Accession NC_014206
Organism Geobacillus sp. C56-T3

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag GC56T3_RS09795 Protein ID WP_011231143.1
Coordinates 1967424..1967753 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GC56T3_RS09800 Protein ID WP_011231142.1
Coordinates 1967753..1968022 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC56T3_RS09770 1963108..1964286 - 1179 WP_013145374.1 acyl-CoA dehydrogenase family protein -
GC56T3_RS09775 1964466..1965209 - 744 WP_013145375.1 NAD-dependent protein deacylase -
GC56T3_RS09780 1965283..1966032 - 750 WP_013145376.1 oxygen-insensitive NADPH nitroreductase -
GC56T3_RS09785 1966128..1966961 - 834 WP_013145377.1 pirin family protein -
GC56T3_RS18635 1967153..1967392 + 240 WP_083778806.1 hypothetical protein -
GC56T3_RS09795 1967424..1967753 - 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GC56T3_RS09800 1967753..1968022 - 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GC56T3_RS09820 1968850..1969905 - 1056 WP_013145378.1 transposase -
GC56T3_RS09825 1970032..1970796 + 765 WP_041468412.1 hypothetical protein -
GC56T3_RS09830 1970810..1971277 + 468 WP_041468414.1 hypothetical protein -
GC56T3_RS09835 1971346..1972326 - 981 WP_013145379.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T24773 WP_011231143.1 NC_014206:c1967753-1967424 [Geobacillus sp. C56-T3]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T24773 NC_014206:c1967753-1967424 [Geobacillus sp. C56-T3]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT24773 WP_011231142.1 NC_014206:c1968022-1967753 [Geobacillus sp. C56-T3]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT24773 NC_014206:c1968022-1967753 [Geobacillus sp. C56-T3]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References