Detailed information of TA system TA024771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2330397..2331375
• Replicon: chromosome
• Accession: NC_014171
• Organism: Bacillus thuringiensis BMB171 (Bacillus thuringiensis serovar)

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: BMB171_RS12060
• Protein ID: WP_000624977.1
• Coordinates: 2330397..2331134 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: BMB171_RS12065
• Protein ID: WP_000237819.1
• Coordinates: 2331247..2331375 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMB171_RS12035	2326010..2326417	+	408	WP_000063587.1	VOC family protein	-
BMB171_RS12040	2326441..2326821	+	381	WP_002025805.1	hypothetical protein	-
BMB171_RS12045	2326979..2328664	-	1686	WP_033693153.1	alpha-keto acid decarboxylase family protein	-
BMB171_RS12050	2328772..2329254	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
BMB171_RS12055	2329421..2330158	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BMB171_RS12060	2330397..2331134	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
BMB171_RS12065	2331247..2331375	+	129	WP_000237819.1	hypothetical protein	Antitoxin
BMB171_RS12070	2331448..2331624	+	177	WP_000852629.1	stage II sporulation protein SB	-
BMB171_RS12075	2331644..2332033	-	390	WP_000713860.1	YxeA family protein	-
BMB171_RS12080	2332245..2333714	+	1470	WP_000287556.1	beta-Ala-His dipeptidase	-
BMB171_RS12085	2333960..2334769	+	810	WP_000678370.1	C39 family peptidase	-
BMB171_RS12090	2334795..2335397	+	603	WP_000517274.1	hypothetical protein	-
BMB171_RS12095	2335641..2335892	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T24771 WP_000624977.1 NC_014171:2330397-2331134 [Bacillus thuringiensis BMB171]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T24771 NC_014171:2330397-2331134 [Bacillus thuringiensis BMB171]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT24771 WP_000237819.1 NC_014171:2331247-2331375 [Bacillus thuringiensis BMB171]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT24771 NC_014171:2331247-2331375 [Bacillus thuringiensis BMB171]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2