Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 503835..504471 Replicon chromosome
Accession NZ_CP098491
Organism Bacillus subtilis strain NB205

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NDK36_RS02600 Protein ID WP_003156187.1
Coordinates 504121..504471 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NDK36_RS02595 Protein ID WP_003225183.1
Coordinates 503835..504116 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NDK36_RS02575 (NDK36_02575) 500194..500793 - 600 WP_014478896.1 rhomboid family intramembrane serine protease -
NDK36_RS02580 (NDK36_02580) 500888..501253 + 366 WP_014478897.1 holo-ACP synthase -
NDK36_RS02585 (NDK36_02585) 501419..502435 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NDK36_RS02590 (NDK36_02590) 502550..503719 + 1170 WP_014478898.1 alanine racemase -
NDK36_RS02595 (NDK36_02595) 503835..504116 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NDK36_RS02600 (NDK36_02600) 504121..504471 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NDK36_RS02605 (NDK36_02605) 504587..505411 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NDK36_RS02610 (NDK36_02610) 505416..505781 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NDK36_RS02615 (NDK36_02615) 505785..506186 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
NDK36_RS02620 (NDK36_02620) 506198..507205 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
NDK36_RS02625 (NDK36_02625) 507274..507603 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NDK36_RS02630 (NDK36_02630) 507600..508082 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NDK36_RS02635 (NDK36_02635) 508048..508836 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NDK36_RS02640 (NDK36_02640) 508836..509435 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T247703 WP_003156187.1 NZ_CP098491:504121-504471 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT247703 WP_003225183.1 NZ_CP098491:503835-504116 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References