Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241939..242581 Replicon chromosome
Accession NC_014171
Organism Bacillus thuringiensis BMB171 (Bacillus thuringiensis serovar)

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BMB171_RS01365 Protein ID WP_000635963.1
Coordinates 242231..242581 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BMB171_RS01360 Protein ID WP_000004570.1
Coordinates 241939..242226 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BMB171_RS01335 237256..238218 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
BMB171_RS01340 238211..238783 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BMB171_RS01345 238877..239236 + 360 WP_000583416.1 holo-ACP synthase -
BMB171_RS01350 239393..240343 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
BMB171_RS01355 240461..241630 + 1170 WP_000390610.1 alanine racemase -
BMB171_RS01360 241939..242226 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BMB171_RS01365 242231..242581 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BMB171_RS01370 242649..244817 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
BMB171_RS01375 244875..244991 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BMB171_RS01380 245187..245645 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T24770 WP_000635963.1 NC_014171:242231-242581 [Bacillus thuringiensis BMB171]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T24770 NC_014171:242231-242581 [Bacillus thuringiensis BMB171]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT24770 WP_000004570.1 NC_014171:241939-242226 [Bacillus thuringiensis BMB171]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT24770 NC_014171:241939-242226 [Bacillus thuringiensis BMB171]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References