Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 985000..985555 Replicon chromosome
Accession NC_014169
Organism Bifidobacterium longum subsp. longum JDM301

Toxin (Protein)


Gene name mazF Uniprot ID D6ZTS1
Locus tag BLJ_RS04095 Protein ID WP_013140587.1
Coordinates 985000..985323 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6ZTS2
Locus tag BLJ_RS04100 Protein ID WP_013140588.1
Coordinates 985310..985555 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLJ_RS10655 (BLJ_0807) 981077..982341 - 1265 Protein_797 Fic family protein -
BLJ_RS04080 (BLJ_0808) 982884..983249 - 366 WP_013140585.1 YccF domain-containing protein -
BLJ_RS04085 (BLJ_0809) 983487..984062 + 576 Protein_799 class I SAM-dependent methyltransferase -
BLJ_RS10660 (BLJ_0810) 984169..984783 + 615 WP_003829107.1 cupin domain-containing protein -
BLJ_RS04095 (BLJ_0811) 985000..985323 - 324 WP_013140587.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BLJ_RS04100 (BLJ_0812) 985310..985555 - 246 WP_013140588.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BLJ_RS04105 (BLJ_0813) 985726..986982 + 1257 WP_003829116.1 HipA domain-containing protein -
BLJ_RS04110 (BLJ_0814) 987405..988676 - 1272 WP_013140589.1 MFS transporter -
BLJ_RS04115 (BLJ_0816) 988833..989495 - 663 WP_038426133.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11801.59 Da        Isoelectric Point: 6.2932

>T24766 WP_013140587.1 NC_014169:c985323-985000 [Bifidobacterium longum subsp. longum JDM301]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVADDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T24766 NC_014169:c985323-985000 [Bifidobacterium longum subsp. longum JDM301]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGCT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGCTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9293.39 Da        Isoelectric Point: 4.7719

>AT24766 WP_013140588.1 NC_014169:c985555-985310 [Bifidobacterium longum subsp. longum JDM301]
MSIQIATRVSDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT24766 NC_014169:c985555-985310 [Bifidobacterium longum subsp. longum JDM301]
ATGAGCATACAGATTGCCACGCGAGTTAGTGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4KDK5

Antitoxin

Source ID Structure
AlphaFold DB A0A087BFX1

References