Detailed information of TA system TA024763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 56220..56809
• Replicon: plasmid pTpau01
• Accession: NC_014159
• Organism: Tsukamurella paurometabola DSM 20162

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D5UZ19
• Locus tag: TPAU_RS21480
• Protein ID: WP_013128848.1
• Coordinates: 56414..56809 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D5UZ18
• Locus tag: TPAU_RS21475
• Protein ID: WP_013128847.1
• Coordinates: 56220..56417 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TPAU_RS21445	51276..51626	+	351	WP_013128842.1	hypothetical protein	-
TPAU_RS21450	51999..52796	+	798	WP_013128843.1	AAA family ATPase	-
TPAU_RS21455	52809..53156	+	348	WP_013128844.1	hypothetical protein	-
TPAU_RS22755	53486..53698	+	213	WP_041945393.1	hypothetical protein	-
TPAU_RS21465	53794..54879	+	1086	WP_115330057.1	tyrosine-type recombinase/integrase	-
TPAU_RS21470	54876..55859	+	984	WP_013128846.1	hypothetical protein	-
TPAU_RS21475	56220..56417	+	198	WP_013128847.1	hypothetical protein	Antitoxin
TPAU_RS21480	56414..56809	+	396	WP_013128848.1	Fic family protein	Toxin
TPAU_RS23280	57070..57246	+	177	WP_013128849.1	hypothetical protein	-
TPAU_RS21485	57715..58137	+	423	WP_013128850.1	hypothetical protein	-
TPAU_RS21490	58257..59021	-	765	WP_041944233.1	hypothetical protein	-
TPAU_RS21495	59070..60374	-	1305	WP_041944585.1	IS256 family transposase	-
TPAU_RS21500	60688..60900	+	213	Protein_61	CoA-disulfide reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99806	99806
	-	flank	IS/Tn	-	-	59070..60380	1310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 13896.50 Da
• Isoelectric Point: 3.9248

>T24763 WP_013128848.1 NC_014159:56414-56809 [Tsukamurella paurometabola DSM 20162]
VTDFPTADEVLIAAEYACGFRPISGDPGLFESSIMRASATVFGQDAYPSVWDKAAALLHSLVTTQALADGNKRTGWAGCW
LLLGLNGYSVSNTLDPDAAEQFVLAIAANELTWEQISARLPEFAAAGQESN

Nucleotide

• Length: 396 bp

>T24763 NC_014159:56414-56809 [Tsukamurella paurometabola DSM 20162]
GTGACCGACTTCCCTACGGCCGATGAGGTTCTGATCGCCGCGGAGTACGCATGCGGATTCAGGCCGATCTCTGGCGACCC
CGGGCTGTTCGAGTCGTCGATCATGCGCGCATCGGCCACAGTGTTCGGACAAGACGCCTACCCGAGCGTGTGGGATAAGG
CGGCCGCCCTGCTGCACTCTCTCGTCACGACCCAGGCACTCGCCGACGGCAACAAGCGAACCGGATGGGCGGGCTGCTGG
CTACTGCTGGGGCTCAACGGATACAGCGTGTCCAACACCCTGGACCCGGATGCTGCGGAACAGTTCGTGCTCGCGATCGC
TGCGAACGAACTCACCTGGGAGCAGATCTCCGCACGGCTCCCCGAGTTCGCTGCGGCTGGGCAAGAATCCAATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7033.98 Da
• Isoelectric Point: 10.6826

>AT24763 WP_013128847.1 NC_014159:56220-56417 [Tsukamurella paurometabola DSM 20162]
MPAMNVEFTEDELAAVRSAASDAGSSMRAFAKQAILDKARNREGLVRAIAARTAERSAELNRRLA

Nucleotide

• Length: 198 bp

>AT24763 NC_014159:56220-56417 [Tsukamurella paurometabola DSM 20162]
ATGCCCGCTATGAACGTGGAGTTCACCGAAGACGAGCTGGCAGCAGTCCGTTCGGCTGCAAGCGACGCCGGATCGTCGAT
GCGAGCTTTCGCGAAGCAAGCGATCCTGGACAAGGCGCGCAATCGGGAGGGGCTGGTCCGAGCAATCGCCGCTCGCACAG
CTGAGCGCTCCGCCGAGCTGAATCGGCGGCTTGCGTGA

Structures

Toxin

• AlphaFold DB: D5UZ19

Antitoxin

• AlphaFold DB: D5UZ18