Detailed information of TA system TA024761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 708388..709015
• Replicon: chromosome
• Accession: NC_014152
• Organism: Thermincola potens JR

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5XCG0
• Locus tag: THERJR_RS03595
• Protein ID: WP_013119607.1
• Coordinates: 708665..709015 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THERJR_RS03590
• Protein ID: WP_013119606.1
• Coordinates: 708388..708663 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THERJR_RS03570	704146..705648	+	1503	WP_013119602.1	alpha-hydroxy-acid oxidizing protein	-
THERJR_RS03575	705661..706104	+	444	WP_013119603.1	4Fe-4S dicluster domain-containing protein	-
THERJR_RS03580	706128..707393	+	1266	WP_013119604.1	NAD(P)/FAD-dependent oxidoreductase	-
THERJR_RS03585	707389..708135	+	747	WP_041587947.1	hypothetical protein	-
THERJR_RS03590	708388..708663	+	276	WP_013119606.1	ribbon-helix-helix protein, CopG family	Antitoxin
THERJR_RS03595	708665..709015	+	351	WP_013119607.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THERJR_RS03600	709183..709911	+	729	WP_013119608.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
THERJR_RS03605	709979..711860	+	1882	Protein_711	RNA-binding transcriptional accessory protein	-
THERJR_RS03610	711983..712807	+	825	WP_013119609.1	hypothetical protein	-
THERJR_RS16730	713000..713149	+	150	WP_198360516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12635.57 Da
• Isoelectric Point: 4.6677

>T24761 WP_013119607.1 NC_014152:708665-709015 [Thermincola potens JR]
MLIRRGDIFFADLSPVVGSEQGGTRPVLVLQNDIGNQYSPTTIVAAITSQISKAKLPTHVEISAKNSGLEKDSVILLEQI
RTIDKSRLMEKVTSLSEEDMEKVAQAIEISLGLIDI

Nucleotide

• Length: 351 bp

>T24761 NC_014152:708665-709015 [Thermincola potens JR]
ATGCTTATCCGACGCGGTGATATCTTCTTTGCAGATTTAAGTCCGGTAGTTGGTTCGGAACAGGGCGGGACGCGACCGGT
TTTAGTACTACAAAACGATATAGGCAATCAATACAGTCCGACCACCATAGTAGCGGCAATCACTTCTCAAATAAGCAAAG
CCAAACTTCCTACTCATGTAGAAATATCTGCAAAAAACAGTGGGTTGGAAAAGGATTCGGTTATTTTGCTGGAACAGATT
CGTACTATTGATAAAAGCCGGTTAATGGAAAAGGTTACATCTTTGAGTGAAGAAGATATGGAAAAGGTAGCTCAAGCTAT
CGAGATCAGCCTAGGGTTAATTGATATTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10574.37 Da
• Isoelectric Point: 9.9603

>AT24761 WP_013119606.1 NC_014152:708388-708663 [Thermincola potens JR]
VAEVKRIMISLPDNLLREVDGIVAAEKLNRSEFIREAMKLYIQEKKRRTIREQMKKGYLEMAKINLALAGENFNVEHEVT
RLFDGRLAEGK

Nucleotide

• Length: 276 bp

>AT24761 NC_014152:708388-708663 [Thermincola potens JR]
GTGGCGGAAGTAAAAAGAATTATGATTAGTTTACCTGATAACCTGTTGCGCGAAGTAGACGGTATCGTGGCTGCGGAAAA
ACTTAATCGCAGTGAGTTTATTCGTGAGGCCATGAAACTTTATATCCAGGAGAAAAAACGGAGAACTATCAGGGAGCAAA
TGAAGAAAGGATACTTAGAGATGGCCAAAATCAACTTGGCGTTGGCTGGAGAAAATTTTAATGTTGAACATGAAGTCACA
CGCCTTTTTGATGGAAGGTTAGCGGAGGGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A0L6W2S7