247590

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1348539..1349455 Replicon chromosome
Accession NZ_CP098417
Organism Bacillus subtilis strain N2-10

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NCL52_RS07045 Protein ID WP_003244695.1
Coordinates 1348709..1349455 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NCL52_RS07040 Protein ID WP_003232646.1
Coordinates 1348539..1348709 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCL52_RS07005 (NCL52_07005) 1345402..1345731 + 330 WP_072176027.1 XkdW family protein -
NCL52_RS07010 (NCL52_07010) 1345728..1345892 + 165 WP_014479563.1 XkdX family protein -
NCL52_RS07015 (NCL52_07015) 1345936..1346775 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
NCL52_RS07020 (NCL52_07020) 1346828..1347097 + 270 WP_003232655.1 hemolysin XhlA family protein -
NCL52_RS07025 (NCL52_07025) 1347110..1347373 + 264 WP_003232653.1 phage holin -
NCL52_RS07030 (NCL52_07030) 1347386..1348279 + 894 WP_122894620.1 N-acetylmuramoyl-L-alanine amidase -
NCL52_RS07035 (NCL52_07035) 1348316..1348453 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NCL52_RS07040 (NCL52_07040) 1348539..1348709 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NCL52_RS07045 (NCL52_07045) 1348709..1349455 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NCL52_RS07050 (NCL52_07050) 1349565..1350566 - 1002 WP_003232642.1 inorganic phosphate transporter -
NCL52_RS07055 (NCL52_07055) 1350579..1351196 - 618 WP_003218470.1 DUF47 domain-containing protein -
NCL52_RS07060 (NCL52_07060) 1351472..1352788 - 1317 WP_085186195.1 serine/threonine exchanger -
NCL52_RS07065 (NCL52_07065) 1353176..1354126 + 951 WP_124059769.1 ring-cleaving dioxygenase -
NCL52_RS07070 (NCL52_07070) 1354227..1354373 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T247590 WP_003244695.1 NZ_CP098417:c1349455-1348709 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT247590 WP_003232646.1 NZ_CP098417:c1348709-1348539 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References