247589

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518757..519393 Replicon chromosome
Accession NZ_CP098417
Organism Bacillus subtilis strain N2-10

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NCL52_RS02660 Protein ID WP_003156187.1
Coordinates 519043..519393 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NCL52_RS02655 Protein ID WP_003225183.1
Coordinates 518757..519038 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCL52_RS02635 (NCL52_02635) 515116..515715 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
NCL52_RS02640 (NCL52_02640) 515810..516175 + 366 WP_014475874.1 holo-ACP synthase -
NCL52_RS02645 (NCL52_02645) 516341..517357 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NCL52_RS02650 (NCL52_02650) 517472..518641 + 1170 WP_017697002.1 alanine racemase -
NCL52_RS02655 (NCL52_02655) 518757..519038 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NCL52_RS02660 (NCL52_02660) 519043..519393 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NCL52_RS02665 (NCL52_02665) 519508..520332 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NCL52_RS02670 (NCL52_02670) 520337..520702 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NCL52_RS02675 (NCL52_02675) 520706..521107 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NCL52_RS02680 (NCL52_02680) 521119..522126 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NCL52_RS02685 (NCL52_02685) 522188..522517 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NCL52_RS02690 (NCL52_02690) 522514..522996 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NCL52_RS02695 (NCL52_02695) 522962..523750 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NCL52_RS02700 (NCL52_02700) 523750..524349 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T247589 WP_003156187.1 NZ_CP098417:519043-519393 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT247589 WP_003225183.1 NZ_CP098417:518757-519038 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References