Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2704026..2704677 Replicon chromosome
Accession NZ_CP098411
Organism Lacticaseibacillus paracasei strain 401

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag NCY29_RS13030 Protein ID WP_003567661.1
Coordinates 2704026..2704409 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag NCY29_RS13035 Protein ID WP_003567665.1
Coordinates 2704429..2704677 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCY29_RS13025 (NCY29_13025) 2703321..2703842 - 522 WP_063558320.1 QueT transporter family protein -
NCY29_RS13030 (NCY29_13030) 2704026..2704409 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NCY29_RS13035 (NCY29_13035) 2704429..2704677 - 249 WP_003567665.1 antitoxin Antitoxin
NCY29_RS13040 (NCY29_13040) 2704745..2705881 - 1137 WP_016369534.1 alanine racemase -
NCY29_RS13045 (NCY29_13045) 2705868..2706242 - 375 WP_003567669.1 holo-ACP synthase -
NCY29_RS13050 (NCY29_13050) 2706412..2707920 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
NCY29_RS13055 (NCY29_13055) 2708046..2708189 - 144 WP_003588978.1 hypothetical protein -
NCY29_RS13060 (NCY29_13060) 2708220..2709608 - 1389 WP_063558321.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T247588 WP_003567661.1 NZ_CP098411:c2704409-2704026 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT247588 WP_003567665.1 NZ_CP098411:c2704677-2704429 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References