Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17449..18092 Replicon plasmid p13001-QIL
Accession NZ_CP098358
Organism Klebsiella pneumoniae strain 13001

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag NB672_RS28285 Protein ID WP_001044770.1
Coordinates 17676..18092 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag NB672_RS28280 Protein ID WP_001261282.1
Coordinates 17449..17679 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB672_RS28235 (NB672_28235) 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
NB672_RS28240 (NB672_28240) 13212..13442 + 231 WP_011977773.1 hypothetical protein -
NB672_RS28245 (NB672_28245) 14334..15116 - 783 WP_004152340.1 site-specific integrase -
NB672_RS28250 (NB672_28250) 15116..15448 - 333 WP_004152339.1 hypothetical protein -
NB672_RS28255 (NB672_28255) 15455..15853 - 399 WP_004171440.1 hypothetical protein -
NB672_RS28260 (NB672_28260) 15879..16208 - 330 WP_004152337.1 hypothetical protein -
NB672_RS28265 (NB672_28265) 16236..16544 - 309 WP_004152336.1 hypothetical protein -
NB672_RS28270 (NB672_28270) 16590..16796 - 207 WP_004153649.1 hypothetical protein -
NB672_RS28275 (NB672_28275) 17031..17492 - 462 WP_072093212.1 hypothetical protein -
NB672_RS28280 (NB672_28280) 17449..17679 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NB672_RS28285 (NB672_28285) 17676..18092 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
NB672_RS28290 (NB672_28290) 18166..18855 + 690 Protein_20 AAA family ATPase -
NB672_RS28295 (NB672_28295) 18910..19607 + 698 WP_223174630.1 IS1 family transposase -
NB672_RS28300 (NB672_28300) 19623..19733 + 111 Protein_22 mercuric transport protein periplasmic component -
NB672_RS28305 (NB672_28305) 19769..20191 + 423 WP_001340589.1 organomercurial transporter MerC -
NB672_RS28310 (NB672_28310) 20243..21937 + 1695 WP_000105636.1 mercury(II) reductase -
NB672_RS28315 (NB672_28315) 21955..22317 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
NB672_RS28320 (NB672_28320) 22314..22550 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / blaKPC-2 - 1..113639 113639
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T247580 WP_001044770.1 NZ_CP098358:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T247580 NZ_CP098358:17676-18092 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT247580 WP_001261282.1 NZ_CP098358:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT247580 NZ_CP098358:17449-17679 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References