Detailed information of TA system TA024757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1150616..1151067
• Replicon: chromosome
• Accession: NC_014150
• Organism: Brachyspira murdochii DSM 12563

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D5U8Q3
• Locus tag: BMUR_RS04950
• Protein ID: WP_013113502.1
• Coordinates: 1150616..1150849 (-)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: D5U8Q4
• Locus tag: BMUR_RS04955
• Protein ID: WP_013113503.1
• Coordinates: 1150846..1151067 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMUR_RS04925	1145683..1146291	-	609	WP_013113497.1	response regulator	-
BMUR_RS04930	1146606..1147676	+	1071	WP_013113498.1	mannose-1-phosphate guanylyltransferase	-
BMUR_RS04935	1147683..1148522	-	840	WP_013113499.1	hypothetical protein	-
BMUR_RS04940	1148535..1149521	-	987	WP_013113500.1	hypothetical protein	-
BMUR_RS04945	1149580..1150449	-	870	WP_013113501.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
BMUR_RS04950	1150616..1150849	-	234	WP_013113502.1	type II toxin-antitoxin system HicA family toxin	Toxin
BMUR_RS04955	1150846..1151067	-	222	WP_013113503.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BMUR_RS04960	1151188..1152777	-	1590	WP_013113504.1	DUF262 domain-containing protein	-
BMUR_RS04965	1153129..1153545	-	417	WP_013113505.1	hypothetical protein	-
BMUR_RS04970	1153558..1154046	-	489	WP_013113506.1	hypothetical protein	-
BMUR_RS04975	1154043..1155230	-	1188	WP_013113507.1	chemotaxis-specific protein-glutamate methyltransferase CheB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-64)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-70)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 9050.73 Da
• Isoelectric Point: 10.1753

>T24757 WP_013113502.1 NC_014150:c1150849-1150616 [Brachyspira murdochii DSM 12563]
MSKLILKDSKTIEKLLLLLGFIKQRQKGSHAFYKHEDGRCTVIPFHSNKVISRPLMRKILNEINLDVEEYNNLIEKI

Nucleotide

• Length: 234 bp

>T24757 NC_014150:c1150849-1150616 [Brachyspira murdochii DSM 12563]
ATGAGTAAGCTAATTTTAAAAGATTCTAAAACTATAGAAAAATTACTGCTTTTATTAGGATTTATTAAACAAAGACAAAA
AGGAAGTCATGCTTTTTATAAACATGAAGATGGAAGATGCACGGTTATTCCTTTTCACTCAAATAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTGATAGAAAAAATATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8554.74 Da
• Isoelectric Point: 4.1276

>AT24757 WP_013113503.1 NC_014150:c1151067-1150846 [Brachyspira murdochii DSM 12563]
MNKKFFAYIEYDKESNVYVGYIPSIVGAHTQADTLEELHIKLKEVLDLCFEEMDNEEKNAIPEFVKIEEIELV

Nucleotide

• Length: 222 bp

>AT24757 NC_014150:c1151067-1150846 [Brachyspira murdochii DSM 12563]
ATGAATAAAAAATTTTTTGCCTATATAGAATATGATAAAGAAAGTAATGTTTATGTTGGATATATTCCTTCAATAGTAGG
TGCTCATACACAGGCTGATACTTTGGAAGAATTGCATATAAAATTAAAAGAAGTTTTGGATTTATGTTTTGAAGAGATGG
ACAATGAAGAGAAGAATGCCATTCCAGAGTTTGTAAAAATAGAAGAAATAGAACTGGTATGA

Structures

Toxin

• AlphaFold DB: D5U8Q3

Antitoxin

• AlphaFold DB: D5U8Q4