Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 160299..160824 Replicon plasmid p52977-166.3
Accession NZ_CP098354
Organism Klebsiella pneumoniae strain 52977_A1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag NB671_RS28860 Protein ID WP_001159868.1
Coordinates 160519..160824 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NB671_RS28855 Protein ID WP_000813634.1
Coordinates 160299..160517 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB671_RS28825 (155376) 155376..155723 + 348 WP_000142449.1 DUF6404 family protein -
NB671_RS28830 (155743) 155743..156333 - 591 WP_000194553.1 hypothetical protein -
NB671_RS28835 (156333) 156333..156590 - 258 WP_000371889.1 hypothetical protein -
NB671_RS28840 (156918) 156918..158021 + 1104 WP_001127573.1 nucleotide-binding protein -
NB671_RS28845 (158051) 158051..159181 + 1131 WP_000542418.1 DUF4238 domain-containing protein -
NB671_RS28850 (159181) 159181..159471 + 291 WP_001229374.1 hypothetical protein -
NB671_RS28855 (160299) 160299..160517 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NB671_RS28860 (160519) 160519..160824 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
NB671_RS28865 (160825) 160825..161631 + 807 WP_001593069.1 site-specific integrase -
NB671_RS28870 (161811) 161811..162455 + 645 WP_001144036.1 ParA family protein -
NB671_RS28875 (162542) 162542..162850 + 309 WP_000030198.1 hypothetical protein -
NB671_RS28885 (163816) 163816..164178 + 363 Protein_177 plasmid segregation protein ParM -
NB671_RS28890 (164171) 164171..164587 + 417 WP_001278813.1 plasmid partitioning/stability family protein -
NB671_RS28895 (164589) 164589..165102 - 514 Protein_179 DUF4113 domain-containing protein -
NB671_RS28900 (165163) 165163..165267 - 105 Protein_180 AAA family ATPase -
NB671_RS28905 (165269) 165269..165592 - 324 Protein_181 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..166337 166337


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T247564 WP_001159868.1 NZ_CP098354:160519-160824 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT247564 WP_000813634.1 NZ_CP098354:160299-160517 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References