Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5422071..5422696 Replicon chromosome
Accession NZ_CP098352
Organism Klebsiella pneumoniae strain 52977_A1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NB671_RS26645 Protein ID WP_002882817.1
Coordinates 5422071..5422454 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NB671_RS26650 Protein ID WP_004150355.1
Coordinates 5422454..5422696 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB671_RS26630 (NB671_26630) 5419437..5420339 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NB671_RS26635 (NB671_26635) 5420336..5420971 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NB671_RS26640 (NB671_26640) 5420968..5421897 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NB671_RS26645 (NB671_26645) 5422071..5422454 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NB671_RS26650 (NB671_26650) 5422454..5422696 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NB671_RS26655 (NB671_26655) 5422901..5423818 + 918 WP_002882812.1 alpha/beta hydrolase -
NB671_RS26660 (NB671_26660) 5423832..5424773 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NB671_RS26665 (NB671_26665) 5424818..5425255 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NB671_RS26670 (NB671_26670) 5425252..5426112 - 861 WP_002882807.1 virulence factor BrkB family protein -
NB671_RS26675 (NB671_26675) 5426106..5426705 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T247552 WP_002882817.1 NZ_CP098352:c5422454-5422071 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT247552 WP_004150355.1 NZ_CP098352:c5422696-5422454 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References