Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4415870..4416651 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A5E1AVC8
Locus tag ECL_RS21480 Protein ID WP_029882967.1
Coordinates 4415870..4416361 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0M7EE97
Locus tag ECL_RS21485 Protein ID WP_013098691.1
Coordinates 4416358..4416651 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS21460 4411610..4412302 + 693 WP_013098687.1 response regulator -
ECL_RS21465 4412401..4414536 - 2136 WP_013098688.1 ornithine decarboxylase -
ECL_RS21470 4414719..4415432 + 714 WP_013098689.1 DUF554 domain-containing protein -
ECL_RS21480 4415870..4416361 - 492 WP_029882967.1 GNAT family N-acetyltransferase Toxin
ECL_RS21485 4416358..4416651 - 294 WP_013098691.1 DUF1778 domain-containing protein Antitoxin
ECL_RS21490 4416900..4418687 - 1788 WP_013098692.1 hybrid sensor histidine kinase/response regulator -
ECL_RS21495 4418684..4419316 - 633 WP_013098693.1 response regulator transcription factor -
ECL_RS21500 4419492..4419806 + 315 WP_013098694.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17629.53 Da        Isoelectric Point: 7.2653

>T24753 WP_029882967.1 NC_014121:c4416361-4415870 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVSGASRTFVCCGNDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLM
ASI

Download         Length: 492 bp

>T24753 NC_014121:c4416361-4415870 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGATCTCTGCCCCAGAGCCCCTTCACGCCGGTCATATTCTTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
TGGCTTATTACTCGCTGGCATCCAGCGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTCGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11005.67 Da        Isoelectric Point: 9.8579

>AT24753 WP_013098691.1 NC_014121:c4416651-4416358 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MPTANTMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQAPAPN
ATLRKTMQTPAPWEREK

Download         Length: 294 bp

>AT24753 NC_014121:c4416651-4416358 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGCCAACTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0M7EE97

References