Detailed information of TA system TA024751

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3792955..3793619
• Replicon: chromosome
• Accession: NC_014121
• Organism: Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ECL_RS18470
• Protein ID: WP_044157892.1
• Coordinates: 3793293..3793619 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0M7FI99
• Locus tag: ECL_RS18465
• Protein ID: WP_007869729.1
• Coordinates: 3792955..3793272 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECL_RS18425	3789034..3789771	+	738	WP_013098169.1	WYL domain-containing protein	-
ECL_RS18430	3789828..3790100	+	273	WP_007869719.1	hypothetical protein	-
ECL_RS18435	3790180..3790440	+	261	WP_007869721.1	hypothetical protein	-
ECL_RS18440	3790567..3791388	+	822	WP_007869723.1	DUF945 domain-containing protein	-
ECL_RS18445	3791412..3791651	+	240	WP_007869725.1	DUF905 domain-containing protein	-
ECL_RS18450	3791755..3792213	+	459	WP_013098170.1	antirestriction protein	-
ECL_RS18455	3792229..3792705	+	477	WP_013098171.1	RadC family protein	-
ECL_RS18460	3792714..3792941	+	228	WP_013098172.1	DUF987 domain-containing protein	-
ECL_RS18465	3792955..3793272	+	318	WP_007869729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECL_RS18470	3793293..3793619	+	327	WP_044157892.1	TA system toxin CbtA family protein	Toxin
ECL_RS18475	3793829..3795208	-	1380	WP_013098174.1	diguanylate cyclase	-
ECL_RS18480	3795519..3796439	+	921	WP_169746048.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
ECL_RS18485	3796797..3796868	+	72	WP_099458937.1	membrane protein YpdK	-
ECL_RS18490	3796924..3798159	-	1236	WP_013098178.1	alanine transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3775404..3793619	18215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12295.27 Da
• Isoelectric Point: 8.9951

>T24751 WP_044157892.1 NC_014121:3793293-3793619 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDPPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Nucleotide

• Length: 327 bp

>T24751 NC_014121:3793293-3793619 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCAGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGGC
GCGTTTGCTGGAACAGCACTACGGTCTGACAATAAACGACCCCCCATTCAGCGATGAAGTTGTTATACAGCAACATATCG
ATGCCGGTATCACCCTGTCAGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
CGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGAGGGCAAGACAGGCAATAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11917.60 Da
• Isoelectric Point: 7.0264

>AT24751 WP_007869729.1 NC_014121:3792955-3793272 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT24751 NC_014121:3792955-3793272 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGACCCACATCATCTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGGCT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGCCGGAAGCTGGATGACACATTCCCAC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M7FI99