Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42325..42968 Replicon plasmid p53015-126.5
Accession NZ_CP098342
Organism Klebsiella pneumoniae strain 53015_G7

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag NB667_RS27520 Protein ID WP_001044770.1
Coordinates 42325..42741 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag NB667_RS27525 Protein ID WP_001261282.1
Coordinates 42738..42968 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB667_RS27485 (NB667_27490) 38735..39004 + 270 WP_000339857.1 hypothetical protein -
NB667_RS27490 (NB667_27495) 39181..40047 - 867 WP_004118283.1 replication initiation protein -
NB667_RS27495 (NB667_27500) 40577..40681 + 105 WP_032409716.1 hypothetical protein -
NB667_RS27500 (NB667_27505) 40810..40908 + 99 Protein_41 hypothetical protein -
NB667_RS27505 (NB667_27510) 41039..41209 - 171 Protein_42 serine hydrolase -
NB667_RS27510 (NB667_27515) 41264..41968 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
NB667_RS27515 (NB667_27520) 42033..42251 - 219 Protein_44 AAA family ATPase -
NB667_RS27520 (NB667_27525) 42325..42741 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
NB667_RS27525 (NB667_27530) 42738..42968 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NB667_RS27530 (NB667_27535) 42925..43385 + 461 Protein_47 hypothetical protein -
NB667_RS27535 (NB667_27540) 43513..43833 + 321 WP_004197770.1 hypothetical protein -
NB667_RS27540 (NB667_27545) 44063..44197 + 135 Protein_49 hypothetical protein -
NB667_RS27545 (NB667_27550) 44225..44554 + 330 WP_004197773.1 hypothetical protein -
NB667_RS27550 (NB667_27555) 44603..45421 + 819 WP_004197776.1 hypothetical protein -
NB667_RS27555 (NB667_27560) 45421..46203 + 783 WP_004197768.1 site-specific integrase -
NB667_RS27560 (NB667_27565) 46370..47332 + 963 WP_004197772.1 Abi family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / ant(3'')-Ia / cmlA1 / aadA2 - 1..126542 126542
- flank IS/Tn - - 41264..41968 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T247480 WP_001044770.1 NZ_CP098342:c42741-42325 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T247480 NZ_CP098342:c42741-42325 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT247480 WP_001261282.1 NZ_CP098342:c42968-42738 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT247480 NZ_CP098342:c42968-42738 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References