Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5404557..5405182 Replicon chromosome
Accession NZ_CP098338
Organism Klebsiella pneumoniae strain 53022_J1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NB666_RS26565 Protein ID WP_002882817.1
Coordinates 5404557..5404940 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NB666_RS26570 Protein ID WP_004150355.1
Coordinates 5404940..5405182 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB666_RS26550 (NB666_26550) 5401923..5402825 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NB666_RS26555 (NB666_26555) 5402822..5403457 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NB666_RS26560 (NB666_26560) 5403454..5404383 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NB666_RS26565 (NB666_26565) 5404557..5404940 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NB666_RS26570 (NB666_26570) 5404940..5405182 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NB666_RS26575 (NB666_26575) 5405387..5406304 + 918 WP_002882812.1 alpha/beta hydrolase -
NB666_RS26580 (NB666_26580) 5406318..5407259 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NB666_RS26585 (NB666_26585) 5407304..5407741 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NB666_RS26590 (NB666_26590) 5407738..5408598 - 861 WP_002882807.1 virulence factor BrkB family protein -
NB666_RS26595 (NB666_26595) 5408592..5409191 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T247463 WP_002882817.1 NZ_CP098338:c5404940-5404557 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT247463 WP_004150355.1 NZ_CP098338:c5405182-5404940 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References